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- W21282337 abstract "This chapter discusses the changes in the content of acetic-alcohol fixable RNA and in enzyme activity that have been followed in meiocyte cytoplasm during the meiotic prophase in Cosmos bipinnatus, Compositae, by quantitative and semiquantitative cytochemical methods. The RNA content of the meiocyte cytoplasm is estimated by cytophotometry of 6 μm sections after pyronin Y or azure B staining. After an initial rise, which may not be significant, the RNA content per meiocyte falls abruptly between zygotene and pachytene to a value less than half of that measured at leptotene. The chapter discusses a study that was conducted in an attempt to detect the changes in enzyme activity that might be associated with ribosome dissolution; for this, the prophase meiocytes of Cosmos was surveyed for lysosome enzymes, particularly acid phosphatase and ribonuclease. The observations on ribonuclease activity were less clear-cut because of higher nonspecific staining and the fact that the procedure gave some reaction in the cell nucleus at all stages. The results show that there are phase-specific changes in cytochemically detectable enzyme activity in the meiocyte cytoplasm during prophase." @default.
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- W21282337 date "1971-01-01" @default.
- W21282337 modified "2023-09-24" @default.
- W21282337 title "Cytoplasmic RNA and Enzyme Activity During the Meiotic Prophase in Cosmos bipinnatus" @default.
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- W21282337 doi "https://doi.org/10.1016/b978-0-408-70149-5.50007-5" @default.
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