FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128314618> ?p ?o ?g. }

• W2128314618 endingPage "2495" @default.
• W2128314618 startingPage "2483" @default.
• W2128314618 abstract "Rap1p binds to sites embedded within the Saccharomyces cerevisiae telomeric TG1-3 tract. Previous studies have led to the hypothesis that Rap1p may recruit Sir3p and Sir3p-associating factors to the telomere. To test this, we tethered Sir3p adjacent to the telomere via LexA binding sites in the rap1-17 mutant that truncates the Rap1p C-terminal 165 amino acids thought to contain sites for Sir3p association. Tethering of LexA-Sir3p adjacent to the telomere is sufficient to restore telomeric silencing, indicating that Sir3p can nucleate silencing at the telomere. Tethering of LexA-Sir3p or the LexA-Sir3p(N2O5) gain-of-function protein to a telomeric LexA site hyperrepresses an adjacent ADE2 gene in wild-type cells. Hence, Sir3p recruitment to the telomere is limiting in telomeric silencing. In addition, LexA-Sir3p(N2O5) hyperrepresses telomeric silencing when tethered to a subtelomeric site 3.6 kb from the telomeric tract. This hyperrepression is dependent on the C terminus of Rap1p, suggesting that subtelomeric LexA-Sir3p(N205) can interact with Rap1p-associated factors at the telomere. We also demonstrate that LexA-Sir3p or LexA-Sir3p(N205) tethered in cis with a short tract of telomeric TG1-3 sequences is sufficient to confer silencing at an internal chromosomal position. Internal silencing is enhanced in rap1-17 strains. We propose that sequestration of silencing factors at the telomere limits the efficiency of internal silencing." @default.
• W2128314618 created "2016-06-24" @default.
• W2128314618 creator A5012132645 @default.
• W2128314618 creator A5034855502 @default.
• W2128314618 creator A5042026897 @default.
• W2128314618 creator A5084993413 @default.
• W2128314618 date "1996-05-01" @default.
• W2128314618 modified "2023-10-16" @default.
• W2128314618 title "Tethered Sir3p Nucleates Silencing at Telomeres and Internal Loci in <i>Saccharomyces cerevisiae</i>" @default.
• W2128314618 cites W15025769 @default.
• W2128314618 cites W1559808711 @default.
• W2128314618 cites W1584515218 @default.
• W2128314618 cites W159748353 @default.
• W2128314618 cites W1598237762 @default.
• W2128314618 cites W1754822275 @default.
• W2128314618 cites W1846058178 @default.
• W2128314618 cites W1964170914 @default.
• W2128314618 cites W1970604907 @default.
• W2128314618 cites W1971227936 @default.
• W2128314618 cites W1975842846 @default.
• W2128314618 cites W1978197643 @default.
• W2128314618 cites W1990186602 @default.
• W2128314618 cites W1991736695 @default.
• W2128314618 cites W1995111407 @default.
• W2128314618 cites W2000737623 @default.
• W2128314618 cites W2006094963 @default.
• W2128314618 cites W2006901859 @default.
• W2128314618 cites W2013554145 @default.
• W2128314618 cites W2015494547 @default.
• W2128314618 cites W2017352502 @default.
• W2128314618 cites W2017670310 @default.
• W2128314618 cites W2018420071 @default.
• W2128314618 cites W2018663197 @default.
• W2128314618 cites W2021432006 @default.
• W2128314618 cites W2028110618 @default.
• W2128314618 cites W2028555609 @default.
• W2128314618 cites W2037164528 @default.
• W2128314618 cites W2039278637 @default.
• W2128314618 cites W2044347050 @default.
• W2128314618 cites W2048684608 @default.
• W2128314618 cites W2056550647 @default.
• W2128314618 cites W2058346978 @default.
• W2128314618 cites W2059594509 @default.
• W2128314618 cites W2063989075 @default.
• W2128314618 cites W2093531054 @default.
• W2128314618 cites W2094922096 @default.
• W2128314618 cites W2124525658 @default.
• W2128314618 cites W2126217681 @default.
• W2128314618 cites W2129709344 @default.
• W2128314618 cites W2146484977 @default.
• W2128314618 cites W2155245588 @default.
• W2128314618 cites W2155722817 @default.
• W2128314618 cites W2163550224 @default.
• W2128314618 cites W2168562183 @default.
• W2128314618 cites W2169333620 @default.
• W2128314618 cites W2170861064 @default.
• W2128314618 doi "https://doi.org/10.1128/mcb.16.5.2483" @default.
• W2128314618 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/231237" @default.
• W2128314618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8628316" @default.
• W2128314618 hasPublicationYear "1996" @default.
• W2128314618 type Work @default.
• W2128314618 sameAs 2128314618 @default.
• W2128314618 citedByCount "68" @default.
• W2128314618 countsByYear W21283146182012 @default.
• W2128314618 countsByYear W21283146182013 @default.
• W2128314618 countsByYear W21283146182014 @default.
• W2128314618 countsByYear W21283146182015 @default.
• W2128314618 countsByYear W21283146182021 @default.
• W2128314618 countsByYear W21283146182023 @default.
• W2128314618 crossrefType "journal-article" @default.
• W2128314618 hasAuthorship W2128314618A5012132645 @default.
• W2128314618 hasAuthorship W2128314618A5034855502 @default.
• W2128314618 hasAuthorship W2128314618A5042026897 @default.
• W2128314618 hasAuthorship W2128314618A5084993413 @default.
• W2128314618 hasBestOaLocation W21283146182 @default.
• W2128314618 hasConcept C104317684 @default.
• W2128314618 hasConcept C119056186 @default.
• W2128314618 hasConcept C158448853 @default.
• W2128314618 hasConcept C177336024 @default.
• W2128314618 hasConcept C202265312 @default.
• W2128314618 hasConcept C23069440 @default.
• W2128314618 hasConcept C2777576037 @default.
• W2128314618 hasConcept C413212 @default.
• W2128314618 hasConcept C54355233 @default.
• W2128314618 hasConcept C86339819 @default.
• W2128314618 hasConcept C86803240 @default.
• W2128314618 hasConcept C94966510 @default.
• W2128314618 hasConcept C95444343 @default.
• W2128314618 hasConceptScore W2128314618C104317684 @default.
• W2128314618 hasConceptScore W2128314618C119056186 @default.
• W2128314618 hasConceptScore W2128314618C158448853 @default.
• W2128314618 hasConceptScore W2128314618C177336024 @default.
• W2128314618 hasConceptScore W2128314618C202265312 @default.
• W2128314618 hasConceptScore W2128314618C23069440 @default.
• W2128314618 hasConceptScore W2128314618C2777576037 @default.
• W2128314618 hasConceptScore W2128314618C413212 @default.
• W2128314618 hasConceptScore W2128314618C54355233 @default.
• W2128314618 hasConceptScore W2128314618C86339819 @default.

• next