Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128443344> ?p ?o ?g. }
- W2128443344 endingPage "2134" @default.
- W2128443344 startingPage "2120" @default.
- W2128443344 abstract "Chromatin-remodeling complexes couple ATP hydrolysis to alterations in histone–DNA interactions and nucleosome mobility, allowing transcription factors access to chromatin. Here, we use triple-helix strand-displacement assays, DNA length-dependent ATPase assays, and DNA-minicircle ATPase assays to establish that RSC, as well as its isolated ATPase subunit Sth1, are DNA translocases. RSC/Sth1 ATPase activity is stimulated by single-stranded DNA, suggesting that Sth1 tracks along one strand of the DNA duplex. Each RSC complex appears to contain a single molecule of Sth1, and isolated Sth1 is capable of nucleosome remodeling. We propose that the remodeling enzyme remains in a fixed position on the octamer and translocates a segment of DNA (with accompanying DNA twist), which breaks histone–DNA contacts and propagates as a wave of DNA around the octamer. The demonstration of DNA translocation presented here provides a mechanistic basis for this DNA wave. To test the relative contribution of twist to remodeling, we use nucleosomes containing nicks in precise locations to uncouple twist and translocation. Nucleosomes bearing nicks are remodeled less efficiently than intact nucleosomes. These results suggest that RSC and Sth1 are DNA translocases that use both DNA translocation and twist to remodel nucleosomes efficiently." @default.
- W2128443344 created "2016-06-24" @default.
- W2128443344 creator A5015217245 @default.
- W2128443344 creator A5020191126 @default.
- W2128443344 creator A5080450210 @default.
- W2128443344 date "2002-08-15" @default.
- W2128443344 modified "2023-10-16" @default.
- W2128443344 title "Chromatin remodeling by RSC involves ATP-dependent DNA translocation" @default.
- W2128443344 cites W1498655641 @default.
- W2128443344 cites W1520588455 @default.
- W2128443344 cites W1546809361 @default.
- W2128443344 cites W1631868599 @default.
- W2128443344 cites W1637308698 @default.
- W2128443344 cites W1766348042 @default.
- W2128443344 cites W1871617923 @default.
- W2128443344 cites W1929568320 @default.
- W2128443344 cites W1963485674 @default.
- W2128443344 cites W1976903076 @default.
- W2128443344 cites W1977876594 @default.
- W2128443344 cites W1978569157 @default.
- W2128443344 cites W1984388559 @default.
- W2128443344 cites W1989968124 @default.
- W2128443344 cites W1990586926 @default.
- W2128443344 cites W1997466838 @default.
- W2128443344 cites W2001650868 @default.
- W2128443344 cites W2002698073 @default.
- W2128443344 cites W2005700199 @default.
- W2128443344 cites W2008878794 @default.
- W2128443344 cites W2011541267 @default.
- W2128443344 cites W2013281906 @default.
- W2128443344 cites W2015472477 @default.
- W2128443344 cites W2018325248 @default.
- W2128443344 cites W2022237004 @default.
- W2128443344 cites W2030542066 @default.
- W2128443344 cites W2030851477 @default.
- W2128443344 cites W2031845110 @default.
- W2128443344 cites W2033325031 @default.
- W2128443344 cites W2044495919 @default.
- W2128443344 cites W2054188881 @default.
- W2128443344 cites W2057894015 @default.
- W2128443344 cites W2059528721 @default.
- W2128443344 cites W2063486767 @default.
- W2128443344 cites W2064114814 @default.
- W2128443344 cites W2064429646 @default.
- W2128443344 cites W2070761003 @default.
- W2128443344 cites W2072421613 @default.
- W2128443344 cites W2075430280 @default.
- W2128443344 cites W2077315884 @default.
- W2128443344 cites W2080278886 @default.
- W2128443344 cites W2081032844 @default.
- W2128443344 cites W2092797711 @default.
- W2128443344 cites W2122571070 @default.
- W2128443344 cites W2123331216 @default.
- W2128443344 cites W2128987469 @default.
- W2128443344 cites W2130749378 @default.
- W2128443344 cites W2135015314 @default.
- W2128443344 cites W2139548989 @default.
- W2128443344 cites W2143658931 @default.
- W2128443344 cites W2144792857 @default.
- W2128443344 cites W2146123529 @default.
- W2128443344 cites W2146281325 @default.
- W2128443344 cites W2147793191 @default.
- W2128443344 cites W2147808841 @default.
- W2128443344 cites W2149676146 @default.
- W2128443344 cites W2154779398 @default.
- W2128443344 cites W2170753025 @default.
- W2128443344 cites W2174021414 @default.
- W2128443344 cites W2180268873 @default.
- W2128443344 cites W2399914936 @default.
- W2128443344 cites W2739174192 @default.
- W2128443344 doi "https://doi.org/10.1101/gad.995002" @default.
- W2128443344 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/186443" @default.
- W2128443344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12183366" @default.
- W2128443344 hasPublicationYear "2002" @default.
- W2128443344 type Work @default.
- W2128443344 sameAs 2128443344 @default.
- W2128443344 citedByCount "229" @default.
- W2128443344 countsByYear W21284433442012 @default.
- W2128443344 countsByYear W21284433442013 @default.
- W2128443344 countsByYear W21284433442014 @default.
- W2128443344 countsByYear W21284433442015 @default.
- W2128443344 countsByYear W21284433442016 @default.
- W2128443344 countsByYear W21284433442017 @default.
- W2128443344 countsByYear W21284433442018 @default.
- W2128443344 countsByYear W21284433442019 @default.
- W2128443344 countsByYear W21284433442020 @default.
- W2128443344 countsByYear W21284433442021 @default.
- W2128443344 countsByYear W21284433442022 @default.
- W2128443344 countsByYear W21284433442023 @default.
- W2128443344 crossrefType "journal-article" @default.
- W2128443344 hasAuthorship W2128443344A5015217245 @default.
- W2128443344 hasAuthorship W2128443344A5020191126 @default.
- W2128443344 hasAuthorship W2128443344A5080450210 @default.
- W2128443344 hasBestOaLocation W21284433441 @default.
- W2128443344 hasConcept C12554922 @default.
- W2128443344 hasConcept C14519417 @default.
- W2128443344 hasConcept C166941617 @default.
- W2128443344 hasConcept C167227067 @default.