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- W2128519306 abstract "SNAREs (soluble N-ethyl maleimide sensitive factor attachment protein receptors) are well-known as membrane fusion machinery in eukaryotic cells. The vesicle-associated v-SNARE engages with its partner t-SNAREs on the target membrane to form 4-helix bundle that bridges two membranes and facilitates fusion. However, during the fusion process the geometric information of vesicles is unveiled due to the resolution limit of conventional light microscopy. Cryo-TEM (Cryogenic Transmission Electron Microscopy) directly shows the geometric information during the membrane fusion, overcoming the resolution limit. Since double vesicles which included a small vesicle inside outer vesicle are observed on the final stage of SNARE driven fusion process, we suggest that dual pathways of yeast minimal-machinery-SNARE driven vesicle fusion are available; 1) developing a single vesicle and 2) developing double vesicle as the final state. Furthemore, Cryo-TEM micrographs shows two kinds of intermediate states; double layers and one layer in the contact area. We guess that the final double vesicles might be developed by passing separate two bilayer contact state, while the final single vesicles might be developed by a fusion process with hemifusion state." @default.
- W2128519306 created "2016-06-24" @default.
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- W2128519306 date "2012-01-01" @default.
- W2128519306 modified "2023-09-29" @default.
- W2128519306 title "Direct Observation of Dual Pathways of Yeast Minimal-Machinery-SNARE Driven Vesicle Fusion" @default.
- W2128519306 doi "https://doi.org/10.1016/j.bpj.2011.11.2730" @default.
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