FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128528051> ?p ?o ?g. }

• W2128528051 endingPage "1262" @default.
• W2128528051 startingPage "1253" @default.
• W2128528051 abstract "Intracellular proteolysis of ingested blood proteins is a crucial physiological process in ticks. In our model tick, Ixodes ricinus, cathepsin L (IrCL1) is part of a gut-associated multi-peptidase complex; its endopeptidase activity is important in the initial phase of haemoglobinolysis. We present the functional and biochemical characterisation of this enzyme. We show, by RNA interference (RNAi), that cathepsin L-like activity that peaks during the slow feeding period of females is associated with IrCL1. Recombinant IrCL1 was expressed in bacteria and yeast. Activity profiling with both peptidyl and physiological protein substrates (haemoglobin and albumin) revealed that IrCL1 is an acidic peptidase with a very low optimum pH (3–4) being unstable above pH 5. This suggests an endo/lysosomal localisation that was confirmed by indirect fluorescence microscopy that immunolocalised IrCL1 inside the vesicles of digestive gut cells. Cleavage specificity determined by a positional scanning synthetic combinatorial library and inhibition profile indicated that IrCL1 has the ligand-binding characteristics of the cathepsin L subfamily of cysteine peptidases. A non-redundant proteolytic function was demonstrated when IrCL1-silenced ticks had a decreased ability to feed compared with controls. The data suggest that IrCL1 may be a promising target against ticks and tick-borne pathogens." @default.
• W2128528051 created "2016-06-24" @default.
• W2128528051 creator A5016211348 @default.
• W2128528051 creator A5022335632 @default.
• W2128528051 creator A5031295914 @default.
• W2128528051 creator A5048776248 @default.
• W2128528051 creator A5049132537 @default.
• W2128528051 creator A5050856602 @default.
• W2128528051 creator A5067990890 @default.
• W2128528051 creator A5068535680 @default.
• W2128528051 creator A5069532325 @default.
• W2128528051 creator A5073259366 @default.
• W2128528051 creator A5074694916 @default.
• W2128528051 creator A5081803641 @default.
• W2128528051 creator A5087001996 @default.
• W2128528051 date "2011-10-01" @default.
• W2128528051 modified "2023-10-17" @default.
• W2128528051 title "IrCL1 – The haemoglobinolytic cathepsin L of the hard tick, Ixodes ricinus" @default.
• W2128528051 cites W1019036297 @default.
• W2128528051 cites W1812339273 @default.
• W2128528051 cites W1904255855 @default.
• W2128528051 cites W1965590460 @default.
• W2128528051 cites W1975917092 @default.
• W2128528051 cites W1976954116 @default.
• W2128528051 cites W1998469878 @default.
• W2128528051 cites W2004440977 @default.
• W2128528051 cites W2007288791 @default.
• W2128528051 cites W2009972285 @default.
• W2128528051 cites W2012845953 @default.
• W2128528051 cites W2014940571 @default.
• W2128528051 cites W2018504907 @default.
• W2128528051 cites W2036868716 @default.
• W2128528051 cites W2038178479 @default.
• W2128528051 cites W2041850755 @default.
• W2128528051 cites W2044163664 @default.
• W2128528051 cites W2045700382 @default.
• W2128528051 cites W2062123506 @default.
• W2128528051 cites W2063800518 @default.
• W2128528051 cites W2067040033 @default.
• W2128528051 cites W2071429505 @default.
• W2128528051 cites W2076848220 @default.
• W2128528051 cites W2078128415 @default.
• W2128528051 cites W2078964667 @default.
• W2128528051 cites W2084172998 @default.
• W2128528051 cites W2089488835 @default.
• W2128528051 cites W2090982370 @default.
• W2128528051 cites W2095290410 @default.
• W2128528051 cites W2098080698 @default.
• W2128528051 cites W2105606854 @default.
• W2128528051 cites W2108244474 @default.
• W2128528051 cites W2110425383 @default.
• W2128528051 cites W2110480775 @default.
• W2128528051 cites W2110761434 @default.
• W2128528051 cites W2113417661 @default.
• W2128528051 cites W2129514462 @default.
• W2128528051 cites W2150840749 @default.
• W2128528051 cites W2156123911 @default.
• W2128528051 cites W2157017502 @default.
• W2128528051 cites W2157318523 @default.
• W2128528051 cites W2157963288 @default.
• W2128528051 cites W2161609934 @default.
• W2128528051 cites W2165786351 @default.
• W2128528051 cites W2172074419 @default.
• W2128528051 doi "https://doi.org/10.1016/j.ijpara.2011.06.006" @default.
• W2128528051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21819989" @default.
• W2128528051 hasPublicationYear "2011" @default.
• W2128528051 type Work @default.
• W2128528051 sameAs 2128528051 @default.
• W2128528051 citedByCount "38" @default.
• W2128528051 countsByYear W21285280512012 @default.
• W2128528051 countsByYear W21285280512013 @default.
• W2128528051 countsByYear W21285280512014 @default.
• W2128528051 countsByYear W21285280512015 @default.
• W2128528051 countsByYear W21285280512016 @default.
• W2128528051 countsByYear W21285280512017 @default.
• W2128528051 countsByYear W21285280512018 @default.
• W2128528051 countsByYear W21285280512019 @default.
• W2128528051 countsByYear W21285280512020 @default.
• W2128528051 countsByYear W21285280512021 @default.
• W2128528051 countsByYear W21285280512022 @default.
• W2128528051 countsByYear W21285280512023 @default.
• W2128528051 crossrefType "journal-article" @default.
• W2128528051 hasAuthorship W2128528051A5016211348 @default.
• W2128528051 hasAuthorship W2128528051A5022335632 @default.
• W2128528051 hasAuthorship W2128528051A5031295914 @default.
• W2128528051 hasAuthorship W2128528051A5048776248 @default.
• W2128528051 hasAuthorship W2128528051A5049132537 @default.
• W2128528051 hasAuthorship W2128528051A5050856602 @default.
• W2128528051 hasAuthorship W2128528051A5067990890 @default.
• W2128528051 hasAuthorship W2128528051A5068535680 @default.
• W2128528051 hasAuthorship W2128528051A5069532325 @default.
• W2128528051 hasAuthorship W2128528051A5073259366 @default.
• W2128528051 hasAuthorship W2128528051A5074694916 @default.
• W2128528051 hasAuthorship W2128528051A5081803641 @default.
• W2128528051 hasAuthorship W2128528051A5087001996 @default.
• W2128528051 hasConcept C153911025 @default.
• W2128528051 hasConcept C159047783 @default.
• W2128528051 hasConcept C159641895 @default.

• next