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• W2128551993 abstract "Background . Insulin directly changes the sheep pleural electrophysiology. The aim of this study was to investigate whether insulin induces similar effects in human pleura, to clarify insulin receptor's involvement, and to demonstrate if glibenclamide (hypoglycemic agent) reverses this effect. Methods . Human parietal pleural specimens were mounted in Ussing chambers. Solutions containing insulin or glibenclamide and insulin with anti-insulin antibody, anti-insulin receptor antibody, and glibenclamide were used. The transmesothelial resistance (<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML><mml:msub><mml:mi>R</mml:mi><mml:mrow><mml:mtext>T</mml:mtext><mml:mtext>M</mml:mtext></mml:mrow></mml:msub></mml:math>) was determined. Immunohistochemistry for the presence of Insulin Receptors (IRa, IRb) was also performed. Results . Insulin increased<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML><mml:msub><mml:mi>R</mml:mi><mml:mrow><mml:mtext>T</mml:mtext><mml:mtext>M</mml:mtext></mml:mrow></mml:msub></mml:math>within 1st min (<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML><mml:mrow><mml:mi>P</mml:mi><mml:mo>=</mml:mo><mml:mn>.016</mml:mn></mml:mrow></mml:math>), when added mesothelially which was inhibited by the anti-insulin and anti-insulin receptor antibodies. Glibenclamide also eliminated the insulin-induced changes. Immunohistochemistry verified the presence of IRa and IRb. Conclusion . Insulin induces electrochemical changes in humans as in sheep via interaction with its receptor. This effect is abolished by glibenclamide." @default.
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• W2128551993 date "2010-01-01" @default.
• W2128551993 modified "2023-09-27" @default.
• W2128551993 title "Insulin-Induced Electrophysiology Changes in Human Pleura Are Mediated via Its Receptor" @default.
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• W2128551993 doi "https://doi.org/10.1155/2010/853176" @default.
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