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• W2128589325 abstract "ABSTRACT It has been proposed that herpes simplex virus 1 with VP22 deleted requires secondary mutation of VHS for viability. Here we show that a replication-competent Δ22 virus constructed by homologous recombination maintains a wild-type (Wt) VHS gene and has no other gross mutations. By contrast, Δ22 viruses recovered from a bacterial artificial chromosome contain multiple amino acid changes within a conserved region of VHS. Hence, the mode of virus rescue influences the acquisition of secondary mutations." @default.
• W2128589325 created "2016-06-24" @default.
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• W2128589325 date "2013-09-15" @default.
• W2128589325 modified "2023-09-27" @default.
• W2128589325 title "Mode of Virus Rescue Determines the Acquisition of VHS Mutations in VP22-Negative Herpes Simplex Virus 1" @default.
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• W2128589325 doi "https://doi.org/10.1128/jvi.01654-13" @default.
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