Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128596604> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2128596604 endingPage "1073" @default.
- W2128596604 startingPage "1068" @default.
- W2128596604 abstract "A novel technology for monitoring the changes of 3,'5'-adenosine cyclic monophosphate (cAMP) in live cells suitable for drug screening relies on the use of cyclic nucleotide-gated channels as biosensors coexpressed with the appropriate target receptor. The technique (termed BD ACTOne) offers measurement of cAMP-dependent calcium influx or membrane depolarization with conventional fluorescent methods both in kinetic and in endpoint modes, optimal for high-throughput and subsequent compound screening. The utility of the technique is reported here based on assay development and high-throughput screening for small-molecule antagonists of the peptide parathyroid hormone 2 receptor (PTH2R). The dual-signaling properties of the receptor were retained in the recombinant system, and the observed pharmacological profile corresponded to data from radiolabeled cAMP determination. The membrane-potential-based high-throughput assay produced reproducible actives and led to the identification of several chemical scaffolds with potential utility as PTH2R ligands." @default.
- W2128596604 created "2016-06-24" @default.
- W2128596604 creator A5018033395 @default.
- W2128596604 creator A5029796080 @default.
- W2128596604 creator A5032756567 @default.
- W2128596604 creator A5048837855 @default.
- W2128596604 creator A5055504968 @default.
- W2128596604 date "2007-12-01" @default.
- W2128596604 modified "2023-10-18" @default.
- W2128596604 title "Application of the BD ACTOne™ Technology for the High-Throughput Screening of Gs-Coupled Receptor Antagonists" @default.
- W2128596604 cites W1991001144 @default.
- W2128596604 cites W2011072178 @default.
- W2128596604 cites W2018943211 @default.
- W2128596604 cites W2034395399 @default.
- W2128596604 cites W2039654095 @default.
- W2128596604 cites W2049727620 @default.
- W2128596604 cites W2142826175 @default.
- W2128596604 cites W2155241846 @default.
- W2128596604 cites W2158713438 @default.
- W2128596604 cites W2165468067 @default.
- W2128596604 cites W4241906225 @default.
- W2128596604 doi "https://doi.org/10.1177/1087057107309035" @default.
- W2128596604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18087071" @default.
- W2128596604 hasPublicationYear "2007" @default.
- W2128596604 type Work @default.
- W2128596604 sameAs 2128596604 @default.
- W2128596604 citedByCount "17" @default.
- W2128596604 countsByYear W21285966042012 @default.
- W2128596604 countsByYear W21285966042013 @default.
- W2128596604 countsByYear W21285966042014 @default.
- W2128596604 countsByYear W21285966042015 @default.
- W2128596604 countsByYear W21285966042016 @default.
- W2128596604 countsByYear W21285966042017 @default.
- W2128596604 countsByYear W21285966042018 @default.
- W2128596604 countsByYear W21285966042019 @default.
- W2128596604 countsByYear W21285966042021 @default.
- W2128596604 crossrefType "journal-article" @default.
- W2128596604 hasAuthorship W2128596604A5018033395 @default.
- W2128596604 hasAuthorship W2128596604A5029796080 @default.
- W2128596604 hasAuthorship W2128596604A5032756567 @default.
- W2128596604 hasAuthorship W2128596604A5048837855 @default.
- W2128596604 hasAuthorship W2128596604A5055504968 @default.
- W2128596604 hasBestOaLocation W21285966041 @default.
- W2128596604 hasConcept C12554922 @default.
- W2128596604 hasConcept C135285700 @default.
- W2128596604 hasConcept C161624437 @default.
- W2128596604 hasConcept C170493617 @default.
- W2128596604 hasConcept C185592680 @default.
- W2128596604 hasConcept C2776668983 @default.
- W2128596604 hasConcept C2776991684 @default.
- W2128596604 hasConcept C4141045 @default.
- W2128596604 hasConcept C51323132 @default.
- W2128596604 hasConcept C55493867 @default.
- W2128596604 hasConcept C74187038 @default.
- W2128596604 hasConcept C86803240 @default.
- W2128596604 hasConceptScore W2128596604C12554922 @default.
- W2128596604 hasConceptScore W2128596604C135285700 @default.
- W2128596604 hasConceptScore W2128596604C161624437 @default.
- W2128596604 hasConceptScore W2128596604C170493617 @default.
- W2128596604 hasConceptScore W2128596604C185592680 @default.
- W2128596604 hasConceptScore W2128596604C2776668983 @default.
- W2128596604 hasConceptScore W2128596604C2776991684 @default.
- W2128596604 hasConceptScore W2128596604C4141045 @default.
- W2128596604 hasConceptScore W2128596604C51323132 @default.
- W2128596604 hasConceptScore W2128596604C55493867 @default.
- W2128596604 hasConceptScore W2128596604C74187038 @default.
- W2128596604 hasConceptScore W2128596604C86803240 @default.
- W2128596604 hasIssue "8" @default.
- W2128596604 hasLocation W21285966041 @default.
- W2128596604 hasLocation W21285966042 @default.
- W2128596604 hasOpenAccess W2128596604 @default.
- W2128596604 hasPrimaryLocation W21285966041 @default.
- W2128596604 hasRelatedWork W1974664442 @default.
- W2128596604 hasRelatedWork W2061312135 @default.
- W2128596604 hasRelatedWork W2073620294 @default.
- W2128596604 hasRelatedWork W2074660549 @default.
- W2128596604 hasRelatedWork W2094311897 @default.
- W2128596604 hasRelatedWork W2158548049 @default.
- W2128596604 hasRelatedWork W2893816517 @default.
- W2128596604 hasRelatedWork W3047080990 @default.
- W2128596604 hasRelatedWork W3112815829 @default.
- W2128596604 hasRelatedWork W4223528211 @default.
- W2128596604 hasVolume "12" @default.
- W2128596604 isParatext "false" @default.
- W2128596604 isRetracted "false" @default.
- W2128596604 magId "2128596604" @default.
- W2128596604 workType "article" @default.