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- W2128701928 abstract "Normal mouse spleen cells cannot make primary antibodies in vitro to the thymus-dependent SRBC antigens if cultivated under conditions where normal homologous serum is the sole serum source, but they can do so to the thymus-independent lipopolysaccharide antigen of E. coli O55:B5 bacteria. Normal mouse spleen cells can make primary antibodies to SRBC if cultivated in normal homologous serum provided that a non-specific T-cell stimulus such as an MLR or exposure to PHA is initiated from the start of the culture period. The same T-cell stimuli will prove inhibitory to the immune responsiveness of spleen cells cultivated in foetal calf serum. Spleen cells cultivated in homologous serum obtained from thymectomized lethally irradiated foetal liver reconstituted donors will respond with a normal proliferative response to SRBC. It is suggested that normal serum contains material which decreases proliferation of homologous lymphoid cells and that material in foetal heter-ologous sera stimulates mouse T-cells to proliferate and secrete soluble factors which enable B-cells to differentiate into high rate antibody-forming cells in vitro." @default.
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- W2128701928 date "1972-10-01" @default.
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- W2128701928 title "Mouse Spleen Cells Cultivated in Homologous Serum Can Make Primary Antibodies in Vitro Provided the T-Cells Are Non-Specifically Activated" @default.
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- W2128701928 doi "https://doi.org/10.1111/j.1365-3083.1972.tb03302.x" @default.
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