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• W2128722019 abstract "Leaves are usually the target tissue for expressing transgenes conferring resistances to herbicides, pests, and diseases. To achieve leaf-specific expression, a light-harvest chlorophyll a/b binding protein ( CAB ) of photosystem-II ( CAB2 ) promoter ( CAB2 -p) from rice ( Oryza sativa L.) and the cauliflower mosaic virus 35S promoter were fused to the β-glucuronidase (GUS) reporter and subsequently evaluated in transgenic sweetpotato [ Ipomoea batatas L. (Lam.)]. The 35S promoter-directed GUS activities varied from 46.0 to 61.2 nmol 4-methyl-umbelliferyl-β-D-glucuronide (4-MU) per minute per milligram of protein in leaf, stem, primary, and storage roots. In contrast, the CAB2 -p directed an uneven distribution of GUS activities (4-MU at 1.1 to 12.6 nmol·min −1 ·mg −1 protein); GUS activity in mature leaves was ≈12-fold as high as that in storage roots. In addition, GUS assay in leaf tissues revealed that CAB2 -p enabled a developmentally controlled and light-regulated GUS expression. These results indicate that the rice CAB2 -p could be used to drive leaf-specific expression of linked genes in sweetpotato." @default.
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• W2128722019 date "2007-07-01" @default.
• W2128722019 modified "2023-09-26" @default.
• W2128722019 title "Expression of a Rice Chlorophyll a/b Binding Protein Promoter in Sweetpotato" @default.
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• W2128722019 doi "https://doi.org/10.21273/jashs.132.4.551" @default.
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