FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128746564> ?p ?o ?g. }

• W2128746564 endingPage "858" @default.
• W2128746564 startingPage "848" @default.
• W2128746564 abstract "The alternative sigma factor σ(E) (rpoE) is essential for survival in vivo of Salmonella Typhimurium but is dispensable during growth in the laboratory. We have been identifying σ(E)-regulated genes and studying their regulation and function to elucidate their potential role in the severe attenuation of S. Typhimurium rpoE mutants. In this study we identify five promoters that control the rseP, yaeT (bamA), skp region. A confirmed σ(E)-dependent promoter, yaeTp1, and a second downstream promoter, yaeTp2, are located within the upstream gene rseP and direct expression of the downstream genes. The only known function of RseP is σ(E) activation, and it is therefore not expected to be essential for S. Typhimurium in vitro. However, it proved impossible to delete the entire rseP gene due to the presence of internal promoters that regulate the essential gene yaeT. We could inactivate rseP by deleting the first third of the gene, leaving the yaeT promoters intact. Like the rpoE mutant, the rseP mutant exhibited severe attenuation in vivo. We were able to delete the entire coding sequence of skp, which encodes a periplasmic chaperone involved in targeting misfolded outer-membrane proteins to the β-barrel assembly machinery. The skp mutant was attenuated in mice after oral and parenteral infection. Virulence could be complemented by providing skp in trans but only by linking it to a heterologous σ(E)-regulated promoter. The reason the skp mutant is attenuated is currently enigmatic, but we know it is not through increased sensitivity to a variety of RpoE-activating host stresses, such as H(2)O(2), polymyxin B and high temperature, or through altered secretion of effector proteins by either the Salmonella pathogenicity island (SPI)-1 or the SPI-2 type III secretion system." @default.
• W2128746564 created "2016-06-24" @default.
• W2128746564 creator A5001186878 @default.
• W2128746564 creator A5032181287 @default.
• W2128746564 creator A5048217695 @default.
• W2128746564 creator A5050962688 @default.
• W2128746564 creator A5060261019 @default.
• W2128746564 creator A5064725097 @default.
• W2128746564 creator A5083896588 @default.
• W2128746564 creator A5086812550 @default.
• W2128746564 creator A5087312658 @default.
• W2128746564 date "2011-03-01" @default.
• W2128746564 modified "2023-10-16" @default.
• W2128746564 title "The periplasmic chaperone Skp is required for successful Salmonella Typhimurium infection in a murine typhoid model" @default.
• W2128746564 cites W1592264528 @default.
• W2128746564 cites W1863386058 @default.
• W2128746564 cites W1968977176 @default.
• W2128746564 cites W1970926641 @default.
• W2128746564 cites W1972222322 @default.
• W2128746564 cites W1975398616 @default.
• W2128746564 cites W1975465001 @default.
• W2128746564 cites W1979613397 @default.
• W2128746564 cites W1980858544 @default.
• W2128746564 cites W1992704705 @default.
• W2128746564 cites W1996060443 @default.
• W2128746564 cites W1996080867 @default.
• W2128746564 cites W2000802512 @default.
• W2128746564 cites W2013882590 @default.
• W2128746564 cites W2019381852 @default.
• W2128746564 cites W2022926885 @default.
• W2128746564 cites W2023206510 @default.
• W2128746564 cites W2035250546 @default.
• W2128746564 cites W2036917159 @default.
• W2128746564 cites W2039555609 @default.
• W2128746564 cites W2041025550 @default.
• W2128746564 cites W2042048278 @default.
• W2128746564 cites W2055341880 @default.
• W2128746564 cites W2067703191 @default.
• W2128746564 cites W2080055250 @default.
• W2128746564 cites W2085200140 @default.
• W2128746564 cites W2085573005 @default.
• W2128746564 cites W2085881545 @default.
• W2128746564 cites W2099460073 @default.
• W2128746564 cites W2105592847 @default.
• W2128746564 cites W2110176907 @default.
• W2128746564 cites W2114511757 @default.
• W2128746564 cites W2116137883 @default.
• W2128746564 cites W2117805957 @default.
• W2128746564 cites W2118603057 @default.
• W2128746564 cites W2118629371 @default.
• W2128746564 cites W2118991108 @default.
• W2128746564 cites W2123784166 @default.
• W2128746564 cites W2124395493 @default.
• W2128746564 cites W2125007814 @default.
• W2128746564 cites W2128078820 @default.
• W2128746564 cites W2129087128 @default.
• W2128746564 cites W2133544541 @default.
• W2128746564 cites W2137846679 @default.
• W2128746564 cites W2149689268 @default.
• W2128746564 cites W2153932901 @default.
• W2128746564 cites W2155016120 @default.
• W2128746564 cites W2159777397 @default.
• W2128746564 cites W2160203432 @default.
• W2128746564 cites W2160208361 @default.
• W2128746564 cites W2163136815 @default.
• W2128746564 cites W2171809976 @default.
• W2128746564 cites W2266909067 @default.
• W2128746564 doi "https://doi.org/10.1099/mic.0.046011-0" @default.
• W2128746564 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21148205" @default.
• W2128746564 hasPublicationYear "2011" @default.
• W2128746564 type Work @default.
• W2128746564 sameAs 2128746564 @default.
• W2128746564 citedByCount "18" @default.
• W2128746564 countsByYear W21287465642012 @default.
• W2128746564 countsByYear W21287465642013 @default.
• W2128746564 countsByYear W21287465642014 @default.
• W2128746564 countsByYear W21287465642017 @default.
• W2128746564 countsByYear W21287465642019 @default.
• W2128746564 countsByYear W21287465642020 @default.
• W2128746564 countsByYear W21287465642021 @default.
• W2128746564 countsByYear W21287465642022 @default.
• W2128746564 crossrefType "journal-article" @default.
• W2128746564 hasAuthorship W2128746564A5001186878 @default.
• W2128746564 hasAuthorship W2128746564A5032181287 @default.
• W2128746564 hasAuthorship W2128746564A5048217695 @default.
• W2128746564 hasAuthorship W2128746564A5050962688 @default.
• W2128746564 hasAuthorship W2128746564A5060261019 @default.
• W2128746564 hasAuthorship W2128746564A5064725097 @default.
• W2128746564 hasAuthorship W2128746564A5083896588 @default.
• W2128746564 hasAuthorship W2128746564A5086812550 @default.
• W2128746564 hasAuthorship W2128746564A5087312658 @default.
• W2128746564 hasBestOaLocation W21287465641 @default.
• W2128746564 hasConcept C101762097 @default.
• W2128746564 hasConcept C104317684 @default.
• W2128746564 hasConcept C142724271 @default.
• W2128746564 hasConcept C143065580 @default.
• W2128746564 hasConcept C150194340 @default.
• W2128746564 hasConcept C153911025 @default.

• next