FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128790762> ?p ?o ?g. }

• W2128790762 endingPage "1750" @default.
• W2128790762 startingPage "1738" @default.
• W2128790762 abstract "The human endometrium regenerates from the lower basalis layer, a germinal compartment that persists after menstruation to give rise to the new upper functionalis layer. Because adult stem cells are present in tissues that undergo regeneration, we hypothesized that human endometrium contains small populations of epithelial and stromal stem cells responsible for cyclical regeneration of endometrial glands and stroma and that these cells would exhibit clonogenicity, a stem-cell property. The aims of this study were to determine 1) the clonogenic activity of human endometrial epithelial and stromal cells, 2) which growth factors support this clonogenic activity, and 3) determine the cellular phenotypes of the clones. Endometrial tissue was obtained from women undergoing hysterectomy. Purified single- cell suspensions of epithelial and stromal cells were cultured at cloning density (300-500/cm(2)) in serum medium or in serum- free medium supplemented with one of eight growth factors. Small numbers of epithelial (0.22%) and stromal cells (1.25%) initiated colonies in serum-containing medium. The majority of colonies were small, containing large, loosely arranged cells, and 37% of epithelial and 1 in 60 of stromal colonies were classified as large, comprising small, densely packed cells. In serum-free medium, transforming growth factor-alpha (TGF alpha), epidermal growth factor (EGF), platelet-derived growth factor-BB (PDGF-BB) strongly supported clonogenicity of epithelial cells, while leukemia-inhibitory factor (LIF), hepatocyte growth factor (HGF), stem-cell factor (SCF), insulin-like growth factor-I (IGF- I) were weakly supportive, and basic fibroblast growth factor (bFGF) was without effect. TGF alpha, EGF, PDGF-BB, and bFGF supported stromal cell clonogenicity, while HGF, SCF, LIF, and IGF- I were without effect. Small epithelial colonies expressed three epithelial markers but not stromal markers; however, large epithelial colonies showed little reactivity for all markers except alpha(6)-integrin. All stromal colonies contained fibroblasts, expressing stromal markers, and in some colonies, myofibroblasts were also identified. This analysis of human endometrium has demonstrated the presence of rare clonogenic epithelial and stromal cells with high proliferative potential, providing the first evidence for the existence of putative endometrial epithelial and stromal stem cells." @default.
• W2128790762 created "2016-06-24" @default.
• W2128790762 creator A5012509613 @default.
• W2128790762 creator A5044939352 @default.
• W2128790762 creator A5064169319 @default.
• W2128790762 date "2004-06-01" @default.
• W2128790762 modified "2023-10-03" @default.
• W2128790762 title "Clonogenicity of Human Endometrial Epithelial and Stromal Cells1" @default.
• W2128790762 cites W126539919 @default.
• W2128790762 cites W1483929886 @default.
• W2128790762 cites W1505671001 @default.
• W2128790762 cites W1514351476 @default.
• W2128790762 cites W1519147104 @default.
• W2128790762 cites W1527203018 @default.
• W2128790762 cites W1555330200 @default.
• W2128790762 cites W1600272283 @default.
• W2128790762 cites W1709402215 @default.
• W2128790762 cites W1968613483 @default.
• W2128790762 cites W1978384704 @default.
• W2128790762 cites W1981200960 @default.
• W2128790762 cites W1984386950 @default.
• W2128790762 cites W1992760653 @default.
• W2128790762 cites W1997716402 @default.
• W2128790762 cites W2001046883 @default.
• W2128790762 cites W2002994277 @default.
• W2128790762 cites W2004052832 @default.
• W2128790762 cites W2005356516 @default.
• W2128790762 cites W2014193660 @default.
• W2128790762 cites W2018372782 @default.
• W2128790762 cites W2041011654 @default.
• W2128790762 cites W2046608680 @default.
• W2128790762 cites W2048009305 @default.
• W2128790762 cites W2049859586 @default.
• W2128790762 cites W2054721944 @default.
• W2128790762 cites W2056199674 @default.
• W2128790762 cites W2059570109 @default.
• W2128790762 cites W2061203898 @default.
• W2128790762 cites W2071994718 @default.
• W2128790762 cites W2073910979 @default.
• W2128790762 cites W2074624875 @default.
• W2128790762 cites W2077107241 @default.
• W2128790762 cites W2098035469 @default.
• W2128790762 cites W2104576151 @default.
• W2128790762 cites W2109064870 @default.
• W2128790762 cites W2119852379 @default.
• W2128790762 cites W2126917358 @default.
• W2128790762 cites W2127617517 @default.
• W2128790762 cites W2130516299 @default.
• W2128790762 cites W2135499528 @default.
• W2128790762 cites W2136826860 @default.
• W2128790762 cites W2141817304 @default.
• W2128790762 cites W2144050333 @default.
• W2128790762 cites W2146697101 @default.
• W2128790762 cites W2151658008 @default.
• W2128790762 cites W2159999899 @default.
• W2128790762 cites W2160882660 @default.
• W2128790762 cites W2165491607 @default.
• W2128790762 cites W2168382597 @default.
• W2128790762 cites W2204640292 @default.
• W2128790762 cites W2344772199 @default.
• W2128790762 cites W2347010436 @default.
• W2128790762 cites W2753462020 @default.
• W2128790762 cites W2928313817 @default.
• W2128790762 doi "https://doi.org/10.1095/biolreprod.103.024109" @default.
• W2128790762 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14766732" @default.
• W2128790762 hasPublicationYear "2004" @default.
• W2128790762 type Work @default.
• W2128790762 sameAs 2128790762 @default.
• W2128790762 citedByCount "539" @default.
• W2128790762 countsByYear W21287907622012 @default.
• W2128790762 countsByYear W21287907622013 @default.
• W2128790762 countsByYear W21287907622014 @default.
• W2128790762 countsByYear W21287907622015 @default.
• W2128790762 countsByYear W21287907622016 @default.
• W2128790762 countsByYear W21287907622017 @default.
• W2128790762 countsByYear W21287907622018 @default.
• W2128790762 countsByYear W21287907622019 @default.
• W2128790762 countsByYear W21287907622020 @default.
• W2128790762 countsByYear W21287907622021 @default.
• W2128790762 countsByYear W21287907622022 @default.
• W2128790762 countsByYear W21287907622023 @default.
• W2128790762 crossrefType "journal-article" @default.
• W2128790762 hasAuthorship W2128790762A5012509613 @default.
• W2128790762 hasAuthorship W2128790762A5044939352 @default.
• W2128790762 hasAuthorship W2128790762A5064169319 @default.
• W2128790762 hasBestOaLocation W21287907621 @default.
• W2128790762 hasConcept C117262875 @default.
• W2128790762 hasConcept C125555471 @default.
• W2128790762 hasConcept C126322002 @default.
• W2128790762 hasConcept C134018914 @default.
• W2128790762 hasConcept C16930146 @default.
• W2128790762 hasConcept C170493617 @default.
• W2128790762 hasConcept C180361614 @default.
• W2128790762 hasConcept C2775960820 @default.
• W2128790762 hasConcept C2776362946 @default.
• W2128790762 hasConcept C2776996007 @default.
• W2128790762 hasConcept C2781294074 @default.
• W2128790762 hasConcept C28328180 @default.

• next