Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128889154> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2128889154 endingPage "6836" @default.
- W2128889154 startingPage "6832" @default.
- W2128889154 abstract "Mesophyll protoplasts of Lycopersicon esculentum were treated with iodoacetamide to inactivate mitochondria, and protoplasts of Solanum acaule and Solanum tuberosum were irradiated with gamma- or x-rays to inactivate nuclei. Mixtures of protoplasts thus modified were treated with Ca2+ and polyethylene glycol to obtain heterologous fusion products. Among the fusion products were some tomato plants that were indistinguishable from the original cultivars with respect to morphology, physiology, and chromosome number (2N = 24) but exhibited various degrees of male sterility (MS): complete lack or malformation of anthers, shrunken pollen, and normal-looking stainable pollen that could not germinate. The MS thus induced in five cultivars of different growth types, including one of subspecies L. esculentum cerasiforme, was inherited maternally over several generations and is, therefore, cytoplasmically determined MS (CMS). Analysis of mitochondrial DNA revealed that the mitochondrial genome of the CMS hybrids does not contain all elements of the mitochondrial DNA of either parent but includes sequences of a recombinational nature not present in either parent. The CMS hybrids, therefore, possess a true hybrid mitochondrial genome. The same procedure applied to fusion of tomato with Solanum lycopersicoides and Nicotiana tabacum cells did not produce CMS phenotypes. The advantages of this method over others for generating MS are as follows: (i) only one step is required; (ii) the nuclear genotype of the cultivar is unaffected; (iii) the prospect that cytoplasmic determination allows generation of 100% CMS progenies. The normal-appearing but nonfunctional pollen of certain CMS types might render them attractive to pollinating bumblebees that thus would facilitate production of hybrid seed." @default.
- W2128889154 created "2016-06-24" @default.
- W2128889154 creator A5001565270 @default.
- W2128889154 creator A5021013766 @default.
- W2128889154 creator A5027610409 @default.
- W2128889154 creator A5054247416 @default.
- W2128889154 creator A5055223726 @default.
- W2128889154 date "1992-08-01" @default.
- W2128889154 modified "2023-09-25" @default.
- W2128889154 title "One-step generation of cytoplasmic male sterility by fusion of mitochondrial-inactivated tomato protoplasts with nuclear-inactivated Solanum protoplasts." @default.
- W2128889154 cites W1987479068 @default.
- W2128889154 cites W2068226669 @default.
- W2128889154 cites W2077384376 @default.
- W2128889154 doi "https://doi.org/10.1073/pnas.89.15.6832" @default.
- W2128889154 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/49598" @default.
- W2128889154 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11607310" @default.
- W2128889154 hasPublicationYear "1992" @default.
- W2128889154 type Work @default.
- W2128889154 sameAs 2128889154 @default.
- W2128889154 citedByCount "69" @default.
- W2128889154 countsByYear W21288891542012 @default.
- W2128889154 countsByYear W21288891542015 @default.
- W2128889154 countsByYear W21288891542016 @default.
- W2128889154 countsByYear W21288891542017 @default.
- W2128889154 countsByYear W21288891542020 @default.
- W2128889154 countsByYear W21288891542021 @default.
- W2128889154 countsByYear W21288891542022 @default.
- W2128889154 countsByYear W21288891542023 @default.
- W2128889154 crossrefType "journal-article" @default.
- W2128889154 hasAuthorship W2128889154A5001565270 @default.
- W2128889154 hasAuthorship W2128889154A5021013766 @default.
- W2128889154 hasAuthorship W2128889154A5027610409 @default.
- W2128889154 hasAuthorship W2128889154A5054247416 @default.
- W2128889154 hasAuthorship W2128889154A5055223726 @default.
- W2128889154 hasBestOaLocation W21288891542 @default.
- W2128889154 hasConcept C104317684 @default.
- W2128889154 hasConcept C134305767 @default.
- W2128889154 hasConcept C173749725 @default.
- W2128889154 hasConcept C20750100 @default.
- W2128889154 hasConcept C24586158 @default.
- W2128889154 hasConcept C2776473034 @default.
- W2128889154 hasConcept C2776687438 @default.
- W2128889154 hasConcept C2777680332 @default.
- W2128889154 hasConcept C2778761015 @default.
- W2128889154 hasConcept C2778803642 @default.
- W2128889154 hasConcept C2780618852 @default.
- W2128889154 hasConcept C2781083041 @default.
- W2128889154 hasConcept C2781205006 @default.
- W2128889154 hasConcept C29172406 @default.
- W2128889154 hasConcept C2993140171 @default.
- W2128889154 hasConcept C48189365 @default.
- W2128889154 hasConcept C54355233 @default.
- W2128889154 hasConcept C55698264 @default.
- W2128889154 hasConcept C59822182 @default.
- W2128889154 hasConcept C86803240 @default.
- W2128889154 hasConceptScore W2128889154C104317684 @default.
- W2128889154 hasConceptScore W2128889154C134305767 @default.
- W2128889154 hasConceptScore W2128889154C173749725 @default.
- W2128889154 hasConceptScore W2128889154C20750100 @default.
- W2128889154 hasConceptScore W2128889154C24586158 @default.
- W2128889154 hasConceptScore W2128889154C2776473034 @default.
- W2128889154 hasConceptScore W2128889154C2776687438 @default.
- W2128889154 hasConceptScore W2128889154C2777680332 @default.
- W2128889154 hasConceptScore W2128889154C2778761015 @default.
- W2128889154 hasConceptScore W2128889154C2778803642 @default.
- W2128889154 hasConceptScore W2128889154C2780618852 @default.
- W2128889154 hasConceptScore W2128889154C2781083041 @default.
- W2128889154 hasConceptScore W2128889154C2781205006 @default.
- W2128889154 hasConceptScore W2128889154C29172406 @default.
- W2128889154 hasConceptScore W2128889154C2993140171 @default.
- W2128889154 hasConceptScore W2128889154C48189365 @default.
- W2128889154 hasConceptScore W2128889154C54355233 @default.
- W2128889154 hasConceptScore W2128889154C55698264 @default.
- W2128889154 hasConceptScore W2128889154C59822182 @default.
- W2128889154 hasConceptScore W2128889154C86803240 @default.
- W2128889154 hasIssue "15" @default.
- W2128889154 hasLocation W21288891541 @default.
- W2128889154 hasLocation W21288891542 @default.
- W2128889154 hasLocation W21288891543 @default.
- W2128889154 hasLocation W21288891544 @default.
- W2128889154 hasOpenAccess W2128889154 @default.
- W2128889154 hasPrimaryLocation W21288891541 @default.
- W2128889154 hasRelatedWork W1982475782 @default.
- W2128889154 hasRelatedWork W1983411722 @default.
- W2128889154 hasRelatedWork W2005750000 @default.
- W2128889154 hasRelatedWork W2013256318 @default.
- W2128889154 hasRelatedWork W2019732052 @default.
- W2128889154 hasRelatedWork W2073496941 @default.
- W2128889154 hasRelatedWork W2077820533 @default.
- W2128889154 hasRelatedWork W2091102281 @default.
- W2128889154 hasRelatedWork W2128889154 @default.
- W2128889154 hasRelatedWork W2333603985 @default.
- W2128889154 hasVolume "89" @default.
- W2128889154 isParatext "false" @default.
- W2128889154 isRetracted "false" @default.
- W2128889154 magId "2128889154" @default.
- W2128889154 workType "article" @default.