Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128913612> ?p ?o ?g. }
- W2128913612 endingPage "2725" @default.
- W2128913612 startingPage "2712" @default.
- W2128913612 abstract "Abstract During mammalian preimplantation development, the cells of the blastocyst's inner cell mass differentiate into the epiblast and primitive endoderm lineages, which give rise to the fetus and extra-embryonic tissues, respectively. Extra-embryonic endoderm (XEN) differentiation can be modeled in vitro by induced expression of GATA transcription factors in mouse embryonic stem cells. Here, we use this GATA-inducible system to quantitatively monitor the dynamics of global proteomic changes during the early stages of this differentiation event and also investigate the fully differentiated phenotype, as represented by embryo-derived XEN cells. Using mass spectrometry-based quantitative proteomic profiling with multivariate data analysis tools, we reproducibly quantified 2,336 proteins across three biological replicates and have identified clusters of proteins characterized by distinct, dynamic temporal abundance profiles. We first used this approach to highlight novel marker candidates of the pluripotent state and XEN differentiation. Through functional annotation enrichment analysis, we have shown that the downregulation of chromatin-modifying enzymes, the reorganization of membrane trafficking machinery, and the breakdown of cell–cell adhesion are successive steps of the extra-embryonic differentiation process. Thus, applying a range of sophisticated clustering approaches to a time-resolved proteomic dataset has allowed the elucidation of complex biological processes which characterize stem cell differentiation and could establish a general paradigm for the investigation of these processes. Stem Cells 2015;33:2712—2725" @default.
- W2128913612 created "2016-06-24" @default.
- W2128913612 creator A5003142918 @default.
- W2128913612 creator A5007256008 @default.
- W2128913612 creator A5009019731 @default.
- W2128913612 creator A5019486463 @default.
- W2128913612 creator A5024762265 @default.
- W2128913612 creator A5025442208 @default.
- W2128913612 creator A5026646873 @default.
- W2128913612 creator A5045266975 @default.
- W2128913612 creator A5058525741 @default.
- W2128913612 creator A5064560423 @default.
- W2128913612 creator A5089100040 @default.
- W2128913612 date "2015-06-23" @default.
- W2128913612 modified "2023-10-12" @default.
- W2128913612 title "Dynamic Proteomic Profiling of Extra-Embryonic Endoderm Differentiation in Mouse Embryonic Stem Cells" @default.
- W2128913612 cites W1519282881 @default.
- W2128913612 cites W1894129931 @default.
- W2128913612 cites W1965754626 @default.
- W2128913612 cites W1974248456 @default.
- W2128913612 cites W1992663780 @default.
- W2128913612 cites W1995706668 @default.
- W2128913612 cites W2011182193 @default.
- W2128913612 cites W2020072016 @default.
- W2128913612 cites W2021030938 @default.
- W2128913612 cites W2022959322 @default.
- W2128913612 cites W2023010129 @default.
- W2128913612 cites W2025192477 @default.
- W2128913612 cites W2026059202 @default.
- W2128913612 cites W2026622296 @default.
- W2128913612 cites W2027870104 @default.
- W2128913612 cites W2035203405 @default.
- W2128913612 cites W2037054531 @default.
- W2128913612 cites W2038695324 @default.
- W2128913612 cites W2044039536 @default.
- W2128913612 cites W2047205409 @default.
- W2128913612 cites W2052790535 @default.
- W2128913612 cites W2057093077 @default.
- W2128913612 cites W2060961033 @default.
- W2128913612 cites W2066792185 @default.
- W2128913612 cites W2068378800 @default.
- W2128913612 cites W2073286325 @default.
- W2128913612 cites W2077765163 @default.
- W2128913612 cites W2086979403 @default.
- W2128913612 cites W2092250528 @default.
- W2128913612 cites W2094444742 @default.
- W2128913612 cites W2103365881 @default.
- W2128913612 cites W2106372318 @default.
- W2128913612 cites W2106839391 @default.
- W2128913612 cites W2107167447 @default.
- W2128913612 cites W2108078422 @default.
- W2128913612 cites W2110052730 @default.
- W2128913612 cites W2110737932 @default.
- W2128913612 cites W2118208715 @default.
- W2128913612 cites W2120330019 @default.
- W2128913612 cites W2121710589 @default.
- W2128913612 cites W2124486107 @default.
- W2128913612 cites W2125695802 @default.
- W2128913612 cites W2129957319 @default.
- W2128913612 cites W2132688454 @default.
- W2128913612 cites W2133500171 @default.
- W2128913612 cites W2141219113 @default.
- W2128913612 cites W2142303444 @default.
- W2128913612 cites W2148455744 @default.
- W2128913612 cites W2157918066 @default.
- W2128913612 cites W2159747138 @default.
- W2128913612 cites W2160697532 @default.
- W2128913612 cites W2166305185 @default.
- W2128913612 cites W2169589032 @default.
- W2128913612 doi "https://doi.org/10.1002/stem.2067" @default.
- W2128913612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26059426" @default.
- W2128913612 hasPublicationYear "2015" @default.
- W2128913612 type Work @default.
- W2128913612 sameAs 2128913612 @default.
- W2128913612 citedByCount "14" @default.
- W2128913612 countsByYear W21289136122015 @default.
- W2128913612 countsByYear W21289136122016 @default.
- W2128913612 countsByYear W21289136122017 @default.
- W2128913612 countsByYear W21289136122018 @default.
- W2128913612 countsByYear W21289136122019 @default.
- W2128913612 countsByYear W21289136122020 @default.
- W2128913612 countsByYear W21289136122022 @default.
- W2128913612 countsByYear W21289136122023 @default.
- W2128913612 crossrefType "journal-article" @default.
- W2128913612 hasAuthorship W2128913612A5003142918 @default.
- W2128913612 hasAuthorship W2128913612A5007256008 @default.
- W2128913612 hasAuthorship W2128913612A5009019731 @default.
- W2128913612 hasAuthorship W2128913612A5019486463 @default.
- W2128913612 hasAuthorship W2128913612A5024762265 @default.
- W2128913612 hasAuthorship W2128913612A5025442208 @default.
- W2128913612 hasAuthorship W2128913612A5026646873 @default.
- W2128913612 hasAuthorship W2128913612A5045266975 @default.
- W2128913612 hasAuthorship W2128913612A5058525741 @default.
- W2128913612 hasAuthorship W2128913612A5064560423 @default.
- W2128913612 hasAuthorship W2128913612A5089100040 @default.
- W2128913612 hasBestOaLocation W21289136121 @default.
- W2128913612 hasConcept C104317684 @default.
- W2128913612 hasConcept C107459253 @default.