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• W2128915028 endingPage "4759" @default.
• W2128915028 startingPage "4753" @default.
• W2128915028 abstract "ABSTRACT Real-time PCR provides a means of detecting and quantifying DNA targets by monitoring PCR product accumulation during cycling as indicated by increased fluorescence. A number of different approaches can be used to generate the fluorescence signal. Three approaches—SYBR Green I (a double-stranded DNA intercalating dye), 5′-exonuclease (enzymatically released fluors), and hybridization probes (fluorescence resonance energy transfer)—were evaluated for use in a real-time PCR assay to detect Brucella abortus . The three assays utilized the same amplification primers to produce an identical amplicon. This amplicon spans a region of the B. abortus genome that includes portions of the alkB gene and the IS 711 insertion element. All three assays were of comparable sensitivity, providing a linear assay over 7 orders of magnitude (from 7.5 ng down to 7.5 fg). However, the greatest specificity was achieved with the hybridization probe assay." @default.
• W2128915028 created "2016-06-24" @default.
• W2128915028 creator A5033035646 @default.
• W2128915028 creator A5079962384 @default.
• W2128915028 creator A5084579733 @default.
• W2128915028 date "2003-08-01" @default.
• W2128915028 modified "2023-10-11" @default.
• W2128915028 title "Real-Time PCR Detection of <i>Brucella abortus</i> : a Comparative Study of SYBR Green I, 5′-Exonuclease, and Hybridization Probe Assays" @default.
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• W2128915028 doi "https://doi.org/10.1128/aem.69.8.4753-4759.2003" @default.
• W2128915028 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/169142" @default.
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• W2128915028 hasPublicationYear "2003" @default.
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