Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129052308> ?p ?o ?g. }
- W2129052308 endingPage "305" @default.
- W2129052308 startingPage "294" @default.
- W2129052308 abstract "Colicin E1 is an E. coli plasmid-encoded water-soluble protein that spontaneously inserts into lipid membranes to form a voltage-gated ion channel. We have employed a novel approach in which site-directed mutagenesis is used to provide highly specific attachment points for nitroxide spin labels. A series of colicin mutants, differing only by the position of a single cysteine residue, were prepared and selectively labeled at that cysteine. A hydrophilic sequence (398-406) within the C-terminal domain of the water-soluble form of the protein was investigated and exhibited an electron paramagnetic resonance (EPR) spectral periodicity strongly suggesting an amphiphilic alpha-helix. After removal of the N-terminus of the protein with trypsin, the spectra for this sequence indicate increased label mobility and a more flexible structure." @default.
- W2129052308 created "2016-06-24" @default.
- W2129052308 creator A5012663688 @default.
- W2129052308 creator A5013899709 @default.
- W2129052308 creator A5024972746 @default.
- W2129052308 creator A5036133609 @default.
- W2129052308 creator A5060990373 @default.
- W2129052308 date "1989-01-01" @default.
- W2129052308 modified "2023-10-18" @default.
- W2129052308 title "Site-directed mutagenesis of colicin E1 provides specific attachment sites for spin labels whose spectra are sensitive to local conformation" @default.
- W2129052308 cites W1509588036 @default.
- W2129052308 cites W1518953398 @default.
- W2129052308 cites W1536888913 @default.
- W2129052308 cites W1549134654 @default.
- W2129052308 cites W1967060446 @default.
- W2129052308 cites W1970492002 @default.
- W2129052308 cites W1973813264 @default.
- W2129052308 cites W1980310183 @default.
- W2129052308 cites W2008045895 @default.
- W2129052308 cites W2016266325 @default.
- W2129052308 cites W2028425784 @default.
- W2129052308 cites W2032719702 @default.
- W2129052308 cites W2059701606 @default.
- W2129052308 cites W2060690425 @default.
- W2129052308 cites W2062704019 @default.
- W2129052308 cites W2067130595 @default.
- W2129052308 cites W2072451526 @default.
- W2129052308 cites W2093689465 @default.
- W2129052308 cites W2147551221 @default.
- W2129052308 cites W2169695708 @default.
- W2129052308 cites W4236909298 @default.
- W2129052308 cites W4243983305 @default.
- W2129052308 doi "https://doi.org/10.1002/prot.340060312" @default.
- W2129052308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2560193" @default.
- W2129052308 hasPublicationYear "1989" @default.
- W2129052308 type Work @default.
- W2129052308 sameAs 2129052308 @default.
- W2129052308 citedByCount "136" @default.
- W2129052308 countsByYear W21290523082012 @default.
- W2129052308 countsByYear W21290523082013 @default.
- W2129052308 countsByYear W21290523082014 @default.
- W2129052308 countsByYear W21290523082015 @default.
- W2129052308 countsByYear W21290523082016 @default.
- W2129052308 countsByYear W21290523082017 @default.
- W2129052308 countsByYear W21290523082018 @default.
- W2129052308 countsByYear W21290523082019 @default.
- W2129052308 countsByYear W21290523082020 @default.
- W2129052308 countsByYear W21290523082021 @default.
- W2129052308 countsByYear W21290523082022 @default.
- W2129052308 countsByYear W21290523082023 @default.
- W2129052308 crossrefType "journal-article" @default.
- W2129052308 hasAuthorship W2129052308A5012663688 @default.
- W2129052308 hasAuthorship W2129052308A5013899709 @default.
- W2129052308 hasAuthorship W2129052308A5024972746 @default.
- W2129052308 hasAuthorship W2129052308A5036133609 @default.
- W2129052308 hasAuthorship W2129052308A5060990373 @default.
- W2129052308 hasConcept C103408237 @default.
- W2129052308 hasConcept C104317684 @default.
- W2129052308 hasConcept C111998727 @default.
- W2129052308 hasConcept C121332964 @default.
- W2129052308 hasConcept C12554922 @default.
- W2129052308 hasConcept C143065580 @default.
- W2129052308 hasConcept C15920480 @default.
- W2129052308 hasConcept C16318435 @default.
- W2129052308 hasConcept C168707992 @default.
- W2129052308 hasConcept C169912494 @default.
- W2129052308 hasConcept C177462420 @default.
- W2129052308 hasConcept C178790620 @default.
- W2129052308 hasConcept C181199279 @default.
- W2129052308 hasConcept C185592680 @default.
- W2129052308 hasConcept C187961010 @default.
- W2129052308 hasConcept C18903297 @default.
- W2129052308 hasConcept C22744801 @default.
- W2129052308 hasConcept C2778530040 @default.
- W2129052308 hasConcept C2779201268 @default.
- W2129052308 hasConcept C2779965526 @default.
- W2129052308 hasConcept C41625074 @default.
- W2129052308 hasConcept C46141821 @default.
- W2129052308 hasConcept C47701112 @default.
- W2129052308 hasConcept C521977710 @default.
- W2129052308 hasConcept C552990157 @default.
- W2129052308 hasConcept C55493867 @default.
- W2129052308 hasConcept C71240020 @default.
- W2129052308 hasConcept C79123411 @default.
- W2129052308 hasConcept C8010536 @default.
- W2129052308 hasConcept C86803240 @default.
- W2129052308 hasConceptScore W2129052308C103408237 @default.
- W2129052308 hasConceptScore W2129052308C104317684 @default.
- W2129052308 hasConceptScore W2129052308C111998727 @default.
- W2129052308 hasConceptScore W2129052308C121332964 @default.
- W2129052308 hasConceptScore W2129052308C12554922 @default.
- W2129052308 hasConceptScore W2129052308C143065580 @default.
- W2129052308 hasConceptScore W2129052308C15920480 @default.
- W2129052308 hasConceptScore W2129052308C16318435 @default.
- W2129052308 hasConceptScore W2129052308C168707992 @default.
- W2129052308 hasConceptScore W2129052308C169912494 @default.
- W2129052308 hasConceptScore W2129052308C177462420 @default.
- W2129052308 hasConceptScore W2129052308C178790620 @default.