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- W2129089609 abstract "A bstract : Using a two‐step cloning strategy, we have synthesized more than 20 variant human fibrinogens for biochemical studies. In preliminary experiments we showed that normal fibrinogen produced in CHO cells serves as an accurate model for plasma fibrinogen. We focus here on those variants whose characterization has provided insight into the mechanism of thrombin‐catalyzed polymerization. Analysis of N‐terminal variants showed that thrombin specificity dictates the ordered release of fibrinopeptides. Nevertheless, analysis of C‐terminal variants indicated that fibrinopeptide B (FpB) release is dependent on polymerization. Changes in the a polymerization site and the high‐affinity calcium‐binding site were associated with a complete loss of polymerization. These experiments showed that alterations in the calcium‐binding site influenced function of the a site; in contrast, alterations in the a site did not alter calcium binding. Analysis of variants in the N‐terminus of the Bβ chain provided the first direct evidence that this region impacts predominantly on lateral aggregation, as has long been presumed. These experiments also suggested that lateral aggregation facilitated by this region proceeds without the release of FpB. From these studies we learned that individual sites within fibrinogen do not function in isolation. We conclude that thrombin‐catalyzed polymerization is mediated by a continuum of concerted interactions." @default.
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- W2129089609 date "2001-06-01" @default.
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- W2129089609 title "Insight from Studies with Recombinant Fibrinogens" @default.
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- W2129089609 doi "https://doi.org/10.1111/j.1749-6632.2001.tb03497.x" @default.
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