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- W2129103664 abstract "The effects of the calcium channel blockers S- and R-verapamil or Ro 40-5967 on penetration processes of peripheral blood lymphocytes (PBL) added to allogenic human vascular endothelial cell (HUVEC) monolayers were studied. Both PBL adhesion and migration were evaluated by combined phase contrast and reflection interference contrast microscopy. PBL adhesion was inhibited by 22% when 100 μg/ml verapamil was added to the cell cultures. PBL migration was completely suppressed with verapamil concentrations above 80 μg/ml (EC50: S-verapamil: 59.7 μg/ml, R-verapamil: 52.3 μg/ml). No differences were seen in the results obtained either with or without cytokine (γ-IFN or IL-1) stimulation. Ro 40-5967 redlfced adhesion completely above concentrations of 40 μg/ml. EC50 for migration was 13 ± 2 or 13 ± 1 μg/ml, respectively. Immunohistochemical analysis of adhesion molecule expression on HUVEC revealed no inhibition of 1CAM-1 and VCAM-1 by verapamil or Ro 40-5967. We concluded that the effects of the calcium channel..." @default.
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- W2129103664 date "1994-01-01" @default.
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- W2129103664 title "Effect of the Calcium Channel Blockers S-, R-Verapamil and Ro 40-5967 on Adhesion and Migration Properties of Lymphocytes Acting on Human Vascular Endothelium" @default.
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- W2129103664 doi "https://doi.org/10.3109/10623329409100968" @default.
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