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- W2129123575 abstract "ABSTRACT Detailed evaluation of gene functions in an asexual fungus requires advanced methods of molecular biology. For the generation of targeted gene deletions in the opportunistic pathogen Aspergillus fumigatus we designed a novel blaster module allowing dominant selection of transformants due to resistance to phleomycin as well as dominant (counter)selection of a Cre recombinase-mediated marker excision event. For validation purposes we have deleted the A. fumigatus pabaA gene in a wild-type isolate by making use of this cassette. The resulting pabaA :: loxP strain served as the recipient for subsequent targeting of the velvet locus. Homologous reconstitution of the deleted gene was performed by an allele whose expression is driven in a nitrogen source-dependent manner, as validated by Northern analyses. Overexpression of the veA locus in A. fumigatus does not result in any obvious phenotype, whereas the sporulation capacities of the veA null mutant are reduced on nitrate-containing medium, a phenotype that is completely restored in the reconstituted strain." @default.
- W2129123575 created "2016-06-24" @default.
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- W2129123575 creator A5057941883 @default.
- W2129123575 creator A5085515135 @default.
- W2129123575 date "2005-07-01" @default.
- W2129123575 modified "2023-09-27" @default.
- W2129123575 title "Deletion and Allelic Exchange of the <i>Aspergillus fumigatus veA</i> Locus via a Novel Recyclable Marker Module" @default.
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- W2129123575 doi "https://doi.org/10.1128/ec.4.7.1298-1307.2005" @default.
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