Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129169304> ?p ?o ?g. }
- W2129169304 endingPage "517" @default.
- W2129169304 startingPage "507" @default.
- W2129169304 abstract "Introducing the Escherichia coli topA20::Tn10 allele to Shigella flexneri results in osmotic sensitivity, a reduced growth rate, an increase in reporter plasmid supercoiling (all common to the E. coli mutants), an inability to grow on MacConkey agar and a loss of virulence gene expression. E. coli mutants harbouring this topA allele often compensate for the loss of DNA topoisomerase l by amplifying the genes coding for topoismerase IV. Unlike the E. coli topA mutants, derivatives of S. flexneri harbouring this topA allele did not appear to acquire any compensatory mutations. We investigated the possibility that this was due in part to an inability of the S. flexneri topoisomerase IV genes to compensate for loss of DNA topoisomerase I when overexpressed. The S. flexneri genes encoding the alpha- and beta subunits of topoisomerase IV were detected and cloned in separate multicopy plasmids. These plasmids complemented well-characterized Salmonella typhimurium temperature-sensitive topoisomerase IV mutations, showing that the S. flexneri and S. typhimurium proteins are capable of combining to form active complexes. When the S. flexneri topoisomerase IV genes were cloned in the same multicopy plasmid and introduced into a S. flexneri topA mutant, the plasmid restored osmotic tolerance, improved the growth rate, allowed growth on MacConkey indicator plates and resulted in a relaxation of reporter plasmid supercoiling. The same plasmid also partially restored S. flexneri virulence gene transcription. These data show that overexpression of the S. flexneri topoisomerase IV genes can compensate for the loss of topoisomerase I in terms of general viability of the cell, DNA supercoiling, and (partially) virulence gene expression. The fact that S. flexneri does not exploit topoisomerase IV gene amplification as E. coli does points to a significant difference in the abilities of these species to adapt to the loss of topoisomerase I." @default.
- W2129169304 created "2016-06-24" @default.
- W2129169304 creator A5009549280 @default.
- W2129169304 creator A5073167063 @default.
- W2129169304 creator A5085557355 @default.
- W2129169304 date "1995-02-01" @default.
- W2129169304 modified "2023-09-25" @default.
- W2129169304 title "Overexpression of the Shigella flexneri genes coding for DNA topoisomerase IV compensates for loss of DNA topoisomerase I: effect on virulence gene expression" @default.
- W2129169304 cites W1495526149 @default.
- W2129169304 cites W1497468021 @default.
- W2129169304 cites W1516666607 @default.
- W2129169304 cites W1557336515 @default.
- W2129169304 cites W1585921788 @default.
- W2129169304 cites W1588664883 @default.
- W2129169304 cites W1592890331 @default.
- W2129169304 cites W1720245305 @default.
- W2129169304 cites W1782700290 @default.
- W2129169304 cites W1876016322 @default.
- W2129169304 cites W1899105578 @default.
- W2129169304 cites W1943807947 @default.
- W2129169304 cites W1955204755 @default.
- W2129169304 cites W1965532529 @default.
- W2129169304 cites W1969244409 @default.
- W2129169304 cites W1971425374 @default.
- W2129169304 cites W1974470327 @default.
- W2129169304 cites W1977406857 @default.
- W2129169304 cites W1978251954 @default.
- W2129169304 cites W1979700870 @default.
- W2129169304 cites W1983452813 @default.
- W2129169304 cites W1985345774 @default.
- W2129169304 cites W1992889417 @default.
- W2129169304 cites W1993971869 @default.
- W2129169304 cites W1996007131 @default.
- W2129169304 cites W2002486322 @default.
- W2129169304 cites W2023364466 @default.
- W2129169304 cites W2027611515 @default.
- W2129169304 cites W2037295768 @default.
- W2129169304 cites W2045710759 @default.
- W2129169304 cites W2056714168 @default.
- W2129169304 cites W2057203470 @default.
- W2129169304 cites W2059096132 @default.
- W2129169304 cites W2061051066 @default.
- W2129169304 cites W2079053054 @default.
- W2129169304 cites W2085743613 @default.
- W2129169304 cites W2097673659 @default.
- W2129169304 cites W2115033904 @default.
- W2129169304 cites W2135099069 @default.
- W2129169304 cites W2139626743 @default.
- W2129169304 cites W2141653906 @default.
- W2129169304 cites W2147746526 @default.
- W2129169304 cites W2148387226 @default.
- W2129169304 cites W2155432444 @default.
- W2129169304 cites W4241591363 @default.
- W2129169304 doi "https://doi.org/10.1111/j.1365-2958.1995.tb02264.x" @default.
- W2129169304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7783621" @default.
- W2129169304 hasPublicationYear "1995" @default.
- W2129169304 type Work @default.
- W2129169304 sameAs 2129169304 @default.
- W2129169304 citedByCount "44" @default.
- W2129169304 countsByYear W21291693042012 @default.
- W2129169304 countsByYear W21291693042013 @default.
- W2129169304 countsByYear W21291693042014 @default.
- W2129169304 countsByYear W21291693042015 @default.
- W2129169304 countsByYear W21291693042016 @default.
- W2129169304 countsByYear W21291693042017 @default.
- W2129169304 countsByYear W21291693042018 @default.
- W2129169304 countsByYear W21291693042019 @default.
- W2129169304 countsByYear W21291693042020 @default.
- W2129169304 countsByYear W21291693042021 @default.
- W2129169304 countsByYear W21291693042023 @default.
- W2129169304 crossrefType "journal-article" @default.
- W2129169304 hasAuthorship W2129169304A5009549280 @default.
- W2129169304 hasAuthorship W2129169304A5073167063 @default.
- W2129169304 hasAuthorship W2129169304A5085557355 @default.
- W2129169304 hasConcept C104317684 @default.
- W2129169304 hasConcept C143065580 @default.
- W2129169304 hasConcept C147897179 @default.
- W2129169304 hasConcept C153911025 @default.
- W2129169304 hasConcept C158456008 @default.
- W2129169304 hasConcept C22744801 @default.
- W2129169304 hasConcept C2781217973 @default.
- W2129169304 hasConcept C54355233 @default.
- W2129169304 hasConcept C547475151 @default.
- W2129169304 hasConcept C552990157 @default.
- W2129169304 hasConcept C60987743 @default.
- W2129169304 hasConcept C73573662 @default.
- W2129169304 hasConcept C86803240 @default.
- W2129169304 hasConcept C89423630 @default.
- W2129169304 hasConceptScore W2129169304C104317684 @default.
- W2129169304 hasConceptScore W2129169304C143065580 @default.
- W2129169304 hasConceptScore W2129169304C147897179 @default.
- W2129169304 hasConceptScore W2129169304C153911025 @default.
- W2129169304 hasConceptScore W2129169304C158456008 @default.
- W2129169304 hasConceptScore W2129169304C22744801 @default.
- W2129169304 hasConceptScore W2129169304C2781217973 @default.
- W2129169304 hasConceptScore W2129169304C54355233 @default.
- W2129169304 hasConceptScore W2129169304C547475151 @default.
- W2129169304 hasConceptScore W2129169304C552990157 @default.