FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129180432> ?p ?o ?g. }

• W2129180432 endingPage "2323" @default.
• W2129180432 startingPage "2309" @default.
• W2129180432 abstract "Erythroid Krüppel-like factor (EKLF) is an essential transcription factor for mammalian β-like globin gene switching, and it specifically activates transcription of the adult β globin gene through binding of its zinc fingers to the promoter. It has been a puzzle that in the mouse, despite its expression throughout the erythroid development, EKLF activates the adult βmaj globin promoter only in erythroid cells beyond the stage of embryonic day 10.5 (E10.5) but not before. We show here that expression of the mouse βmaj globin gene in the aorta-gonad-mesonephros region of E10.5 embryos and in the E14.5 fetal liver is accompanied by predominantly nuclear localization of EKLF. In contrast, EKLF is mainly cytoplasmic in the erythroid cells of E9.5 blood islands in which βmaj is silenced. Remarkably, in a cultured mouse adult erythroleukemic (MEL) cell line, the activation of the βmaj globin gene by dimethyl sulfoxide (DMSO) or hexamethylene-bis-acetamide (HMBA) induction is also paralleled by a shift of the subcellular location of EKLF from the cytoplasm to the nucleus. Blockage of the nuclear import of EKLF in DMSO-induced MEL cells with a nuclear export inhibitor repressed the transcription of the βmaj globin gene. Transient transfection experiments further indicated that the full-sequence context of EKLF was required for the regulation of its subcellular locations in MEL cells during DMSO induction. Finally, in both the E14.5 fetal liver cells and induced MEL cells, the β-like globin locus is colocalized the PML oncogene domain nuclear body, and concentrated with EKLF, RNA polymerase II, and the splicing factor SC35. These data together provide the first evidence that developmental stage- and differentiation state-specific regulation of the nuclear transport of EKLF might be one of the steps necessary for the switch-on of the mammalian adult β globin gene transcription." @default.
• W2129180432 created "2016-06-24" @default.
• W2129180432 creator A5023768530 @default.
• W2129180432 creator A5036743801 @default.
• W2129180432 creator A5041395440 @default.
• W2129180432 creator A5050230000 @default.
• W2129180432 creator A5051241132 @default.
• W2129180432 creator A5053134216 @default.
• W2129180432 creator A5059832393 @default.
• W2129180432 creator A5087381980 @default.
• W2129180432 date "2007-03-01" @default.
• W2129180432 modified "2023-10-18" @default.
• W2129180432 title "Subcellular Transport of EKLF and Switch-On of Murine Adult β_maj Globin Gene Transcription" @default.
• W2129180432 cites W1484529414 @default.
• W2129180432 cites W1535444616 @default.
• W2129180432 cites W1569503463 @default.
• W2129180432 cites W1928245765 @default.
• W2129180432 cites W1937332911 @default.
• W2129180432 cites W1969230311 @default.
• W2129180432 cites W1973049010 @default.
• W2129180432 cites W1974247326 @default.
• W2129180432 cites W1977409466 @default.
• W2129180432 cites W1980590293 @default.
• W2129180432 cites W1990228346 @default.
• W2129180432 cites W1993398475 @default.
• W2129180432 cites W1994838087 @default.
• W2129180432 cites W1998360443 @default.
• W2129180432 cites W1998607972 @default.
• W2129180432 cites W2000475723 @default.
• W2129180432 cites W2003918839 @default.
• W2129180432 cites W2008901305 @default.
• W2129180432 cites W2018331371 @default.
• W2129180432 cites W2036324043 @default.
• W2129180432 cites W2042745425 @default.
• W2129180432 cites W2050956430 @default.
• W2129180432 cites W2052852865 @default.
• W2129180432 cites W2060255615 @default.
• W2129180432 cites W2060929560 @default.
• W2129180432 cites W2062031128 @default.
• W2129180432 cites W2065854842 @default.
• W2129180432 cites W2071000969 @default.
• W2129180432 cites W2074976063 @default.
• W2129180432 cites W2076142853 @default.
• W2129180432 cites W2082881281 @default.
• W2129180432 cites W2083397383 @default.
• W2129180432 cites W2083403694 @default.
• W2129180432 cites W2083911368 @default.
• W2129180432 cites W2085906188 @default.
• W2129180432 cites W2087660940 @default.
• W2129180432 cites W2088928744 @default.
• W2129180432 cites W2092324010 @default.
• W2129180432 cites W2095642631 @default.
• W2129180432 cites W2098609903 @default.
• W2129180432 cites W2109264912 @default.
• W2129180432 cites W2110150849 @default.
• W2129180432 cites W2112715309 @default.
• W2129180432 cites W2114197774 @default.
• W2129180432 cites W2116998164 @default.
• W2129180432 cites W2118511790 @default.
• W2129180432 cites W2119375414 @default.
• W2129180432 cites W2127217227 @default.
• W2129180432 cites W2131382540 @default.
• W2129180432 cites W2137854193 @default.
• W2129180432 cites W2141340108 @default.
• W2129180432 cites W2150519389 @default.
• W2129180432 cites W2167963503 @default.
• W2129180432 cites W2169334713 @default.
• W2129180432 cites W2171393347 @default.
• W2129180432 cites W2171442811 @default.
• W2129180432 cites W2349905826 @default.
• W2129180432 cites W2404741217 @default.
• W2129180432 cites W2411291059 @default.
• W2129180432 cites W2736868935 @default.
• W2129180432 cites W4255110190 @default.
• W2129180432 doi "https://doi.org/10.1128/mcb.01875-06" @default.
• W2129180432 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1820495" @default.
• W2129180432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17242208" @default.
• W2129180432 hasPublicationYear "2007" @default.
• W2129180432 type Work @default.
• W2129180432 sameAs 2129180432 @default.
• W2129180432 citedByCount "16" @default.
• W2129180432 countsByYear W21291804322012 @default.
• W2129180432 countsByYear W21291804322013 @default.
• W2129180432 countsByYear W21291804322014 @default.
• W2129180432 countsByYear W21291804322015 @default.
• W2129180432 countsByYear W21291804322016 @default.
• W2129180432 countsByYear W21291804322019 @default.
• W2129180432 countsByYear W21291804322020 @default.
• W2129180432 countsByYear W21291804322021 @default.
• W2129180432 crossrefType "journal-article" @default.
• W2129180432 hasAuthorship W2129180432A5023768530 @default.
• W2129180432 hasAuthorship W2129180432A5036743801 @default.
• W2129180432 hasAuthorship W2129180432A5041395440 @default.
• W2129180432 hasAuthorship W2129180432A5050230000 @default.
• W2129180432 hasAuthorship W2129180432A5051241132 @default.
• W2129180432 hasAuthorship W2129180432A5053134216 @default.
• W2129180432 hasAuthorship W2129180432A5059832393 @default.
• W2129180432 hasAuthorship W2129180432A5087381980 @default.

• next