Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129279098> ?p ?o ?g. }
- W2129279098 endingPage "2945" @default.
- W2129279098 startingPage "2935" @default.
- W2129279098 abstract "Heat shock induces protein aggregation in Escherichia coli and E. coli (λc1857). The aggregates (S fraction) appear 15 min post-induction and are separable from membranes by sucrose density-gradient centrifugation. The S fraction quickly disappears in wild type strains but persists in rpoH mutant with concomitant quick inner membrane destruction. We propose that: (1) the disappearance of the S fraction reflects a rpoH-dependent processing, (2) the membrane destruction explains the lethality of the rpoH mutation at elevated temperatures; and (3) the protection of the inner membrane integrity is an important physiological function of the heat-shock response. We assume that the S fraction of aggregated proteins represents the signal inducing the heat-shock response. The prophage thermo-induction results in an increase (35 min post-induction) in the A fraction resembling that of the adhesion zones of the membranes. This fraction is greater than the corresponding fraction from uninduced cells. The increase is mediated by the λ late genes, since it is absent in the induced E.coli (λc1857 Oam21). Since heat shock is widely used for induction of the λ promoters in expression vectors it is possible that the formation of the protein aggregates (though transient in WT strains) and/or the fragility of membranes in rpoH mutants may be the cause of poor expression of cloned genes or may lead to mistaken localization of their expression products." @default.
- W2129279098 created "2016-06-24" @default.
- W2129279098 creator A5033497697 @default.
- W2129279098 creator A5044440565 @default.
- W2129279098 creator A5049932288 @default.
- W2129279098 date "1991-12-01" @default.
- W2129279098 modified "2023-10-01" @default.
- W2129279098 title "Response of Escherichia coli cell membranes to induction of ? c1857 prophage by heat shock" @default.
- W2129279098 cites W107673453 @default.
- W2129279098 cites W1481181027 @default.
- W2129279098 cites W1525754348 @default.
- W2129279098 cites W1528870636 @default.
- W2129279098 cites W1531454475 @default.
- W2129279098 cites W1559858723 @default.
- W2129279098 cites W1562400216 @default.
- W2129279098 cites W1575935179 @default.
- W2129279098 cites W1576513219 @default.
- W2129279098 cites W1577674984 @default.
- W2129279098 cites W1587194145 @default.
- W2129279098 cites W1605604337 @default.
- W2129279098 cites W1626622124 @default.
- W2129279098 cites W1645444471 @default.
- W2129279098 cites W1649485376 @default.
- W2129279098 cites W1658751919 @default.
- W2129279098 cites W1875474122 @default.
- W2129279098 cites W188831480 @default.
- W2129279098 cites W1966143901 @default.
- W2129279098 cites W1975352932 @default.
- W2129279098 cites W1979846930 @default.
- W2129279098 cites W1986565420 @default.
- W2129279098 cites W2008072583 @default.
- W2129279098 cites W2014116896 @default.
- W2129279098 cites W2033599126 @default.
- W2129279098 cites W2036794046 @default.
- W2129279098 cites W2040960801 @default.
- W2129279098 cites W2042985028 @default.
- W2129279098 cites W2043579359 @default.
- W2129279098 cites W2057431308 @default.
- W2129279098 cites W2065762927 @default.
- W2129279098 cites W2079839032 @default.
- W2129279098 cites W2085717873 @default.
- W2129279098 cites W2088950300 @default.
- W2129279098 cites W2100837269 @default.
- W2129279098 cites W2128635872 @default.
- W2129279098 cites W2133142747 @default.
- W2129279098 cites W2136345418 @default.
- W2129279098 cites W2141759786 @default.
- W2129279098 cites W2149915307 @default.
- W2129279098 cites W2154715482 @default.
- W2129279098 cites W2157924633 @default.
- W2129279098 cites W2169730471 @default.
- W2129279098 cites W2174167386 @default.
- W2129279098 cites W220070148 @default.
- W2129279098 cites W2314761403 @default.
- W2129279098 cites W4255236900 @default.
- W2129279098 cites W939168437 @default.
- W2129279098 doi "https://doi.org/10.1111/j.1365-2958.1991.tb01853.x" @default.
- W2129279098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1839744" @default.
- W2129279098 hasPublicationYear "1991" @default.
- W2129279098 type Work @default.
- W2129279098 sameAs 2129279098 @default.
- W2129279098 citedByCount "42" @default.
- W2129279098 countsByYear W21292790982012 @default.
- W2129279098 countsByYear W21292790982013 @default.
- W2129279098 countsByYear W21292790982014 @default.
- W2129279098 countsByYear W21292790982016 @default.
- W2129279098 countsByYear W21292790982023 @default.
- W2129279098 crossrefType "journal-article" @default.
- W2129279098 hasAuthorship W2129279098A5033497697 @default.
- W2129279098 hasAuthorship W2129279098A5044440565 @default.
- W2129279098 hasAuthorship W2129279098A5049932288 @default.
- W2129279098 hasConcept C104317684 @default.
- W2129279098 hasConcept C137984847 @default.
- W2129279098 hasConcept C143065580 @default.
- W2129279098 hasConcept C146587185 @default.
- W2129279098 hasConcept C153911025 @default.
- W2129279098 hasConcept C205260736 @default.
- W2129279098 hasConcept C2776441376 @default.
- W2129279098 hasConcept C2778106830 @default.
- W2129279098 hasConcept C41625074 @default.
- W2129279098 hasConcept C46371251 @default.
- W2129279098 hasConcept C547475151 @default.
- W2129279098 hasConcept C55493867 @default.
- W2129279098 hasConcept C65871279 @default.
- W2129279098 hasConcept C73445445 @default.
- W2129279098 hasConcept C7623868 @default.
- W2129279098 hasConcept C86803240 @default.
- W2129279098 hasConcept C95444343 @default.
- W2129279098 hasConceptScore W2129279098C104317684 @default.
- W2129279098 hasConceptScore W2129279098C137984847 @default.
- W2129279098 hasConceptScore W2129279098C143065580 @default.
- W2129279098 hasConceptScore W2129279098C146587185 @default.
- W2129279098 hasConceptScore W2129279098C153911025 @default.
- W2129279098 hasConceptScore W2129279098C205260736 @default.
- W2129279098 hasConceptScore W2129279098C2776441376 @default.
- W2129279098 hasConceptScore W2129279098C2778106830 @default.
- W2129279098 hasConceptScore W2129279098C41625074 @default.
- W2129279098 hasConceptScore W2129279098C46371251 @default.