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• W2129335218 abstract "It is theoretically possible to obtain a catalytic site of an artificial ribozyme from a random sequence consisting of a limited numbers of nucleotides. However, this strategy has been inadequately explored. Here, we report an in vitro selection technique that exploits modular construction of a structurally constrained RNA to acquire a catalytic site for RNA ligation from a short random sequence. To practice the selection, a sequence of 30 nucleotides was located close to the putative reaction site in a derivative of a naturally occurring self-folding RNA whose crystal structure is known. RNAs whose activity depended on the starting three-dimensional structure were selected with 3′–5′ ligation specificity, indicating that the strategy can be used to acquire a variety of catalytic sites and other functional RNA modules." @default.
• W2129335218 created "2016-06-24" @default.
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• W2129335218 date "2004-11-03" @default.
• W2129335218 modified "2023-09-23" @default.
• W2129335218 title "Generation of a catalytic module on a self-folding RNA" @default.
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• W2129335218 doi "https://doi.org/10.1261/rna.7170304" @default.
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