Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129380902> ?p ?o ?g. }
- W2129380902 abstract "Abstract Background The first telomerase-associated protein (Est1) was isolated in yeast due to its essential role in telomere maintenance. The human counterparts EST1A, EST1B, and EST1C perform diverse functions in nonsense-mediated mRNA decay (NMD), telomere length homeostasis, and telomere transcription. Although Est1 and EST1A/B interact with the catalytic subunit of yeast and human telomerase (Est2 and TERT, respectively), the molecular determinants of these interactions have not been elaborated fully. Results To investigate the functional conservation of the EST1 protein family, we performed protein-protein interaction mapping and structure-function analysis. The domain in hEST1A most conserved between species, containing a TPR (tricotetrapeptide repeat), was sufficient for interaction of hEST1A with multiple fragments of hTERT including the N-terminus. Two mutations within the hTERT N-terminus that perturb in vivo function (NAAIRS 92 , NAAIRS 122 ) did not affect this protein interaction. ScEst1 hybrids containing the TPR of hEST1A, hEST1B, or hEST1C were expressed in yeast strains lacking EST1 , yet they failed to complement senescence. Point mutations within and outside the cognate ScEst1 TPR, chosen to disrupt a putative protein interaction surface, resulted in telomere lengthening or shortening without affecting recruitment to telomeres. Conclusions These results identify a domain encompassing the TPR of hEST1A as an hTERT interaction module. The TPR of S. cerevisiae Est1 is required for telomerase-mediated telomere length maintenance in a manner that appears separable from telomere recruitment. Discrete residues in or adjacent to the TPR of Est1 also regulate telomere length homeostasis." @default.
- W2129380902 created "2016-06-24" @default.
- W2129380902 creator A5029541779 @default.
- W2129380902 creator A5032127149 @default.
- W2129380902 creator A5036451569 @default.
- W2129380902 creator A5042093229 @default.
- W2129380902 creator A5050966425 @default.
- W2129380902 date "2011-10-18" @default.
- W2129380902 modified "2023-10-16" @default.
- W2129380902 title "The TPR-containing domain within Est1 homologs exhibits species-specific roles in telomerase interaction and telomere length homeostasis" @default.
- W2129380902 cites W1209714687 @default.
- W2129380902 cites W1547269929 @default.
- W2129380902 cites W1582225942 @default.
- W2129380902 cites W1594622192 @default.
- W2129380902 cites W160809916 @default.
- W2129380902 cites W1918680390 @default.
- W2129380902 cites W1943069336 @default.
- W2129380902 cites W1948628402 @default.
- W2129380902 cites W1956123188 @default.
- W2129380902 cites W1964734962 @default.
- W2129380902 cites W1974377132 @default.
- W2129380902 cites W1976309051 @default.
- W2129380902 cites W1977871027 @default.
- W2129380902 cites W1979164620 @default.
- W2129380902 cites W1980681058 @default.
- W2129380902 cites W1983823417 @default.
- W2129380902 cites W1985645826 @default.
- W2129380902 cites W1988669405 @default.
- W2129380902 cites W1994495961 @default.
- W2129380902 cites W1997132321 @default.
- W2129380902 cites W1999058501 @default.
- W2129380902 cites W2001607785 @default.
- W2129380902 cites W2004869134 @default.
- W2129380902 cites W2015047103 @default.
- W2129380902 cites W2016672919 @default.
- W2129380902 cites W2016712010 @default.
- W2129380902 cites W2019705337 @default.
- W2129380902 cites W2022920512 @default.
- W2129380902 cites W2024039709 @default.
- W2129380902 cites W2030215473 @default.
- W2129380902 cites W2031936092 @default.
- W2129380902 cites W2032149581 @default.
- W2129380902 cites W2038223966 @default.
- W2129380902 cites W2038383843 @default.
- W2129380902 cites W2039574035 @default.
- W2129380902 cites W2040167235 @default.
- W2129380902 cites W2040204581 @default.
- W2129380902 cites W2040265375 @default.
- W2129380902 cites W2041299751 @default.
- W2129380902 cites W2045448955 @default.
- W2129380902 cites W2047017978 @default.
- W2129380902 cites W2049992533 @default.
- W2129380902 cites W2052740988 @default.
- W2129380902 cites W2052786131 @default.
- W2129380902 cites W2053496633 @default.
- W2129380902 cites W2055617420 @default.
- W2129380902 cites W2055707604 @default.
- W2129380902 cites W2057469416 @default.
- W2129380902 cites W2065766937 @default.
- W2129380902 cites W2073204556 @default.
- W2129380902 cites W2081477672 @default.
- W2129380902 cites W2084715034 @default.
- W2129380902 cites W2084894372 @default.
- W2129380902 cites W2087177055 @default.
- W2129380902 cites W2088206409 @default.
- W2129380902 cites W2089777080 @default.
- W2129380902 cites W2091512138 @default.
- W2129380902 cites W2092131372 @default.
- W2129380902 cites W2097876557 @default.
- W2129380902 cites W2098720917 @default.
- W2129380902 cites W2099991915 @default.
- W2129380902 cites W2100291823 @default.
- W2129380902 cites W2100364954 @default.
- W2129380902 cites W2102581124 @default.
- W2129380902 cites W2103148784 @default.
- W2129380902 cites W2109089733 @default.
- W2129380902 cites W2109975152 @default.
- W2129380902 cites W2111723163 @default.
- W2129380902 cites W2113168077 @default.
- W2129380902 cites W2125194621 @default.
- W2129380902 cites W2128940137 @default.
- W2129380902 cites W2130283365 @default.
- W2129380902 cites W2130525093 @default.
- W2129380902 cites W2130628675 @default.
- W2129380902 cites W2131581447 @default.
- W2129380902 cites W2135055810 @default.
- W2129380902 cites W2141157651 @default.
- W2129380902 cites W2141288561 @default.
- W2129380902 cites W2142087742 @default.
- W2129380902 cites W2142605524 @default.
- W2129380902 cites W2143022342 @default.
- W2129380902 cites W2144417959 @default.
- W2129380902 cites W2145554588 @default.
- W2129380902 cites W2147161726 @default.
- W2129380902 cites W2148097975 @default.
- W2129380902 cites W2149159248 @default.
- W2129380902 cites W2149471694 @default.
- W2129380902 cites W2152508214 @default.
- W2129380902 cites W2153744932 @default.
- W2129380902 cites W2159430769 @default.