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• W2129397265 abstract "Putative U6snRNA polymerase III (PolIII) promoters were cloned from the Anopheles gambiae and Aedes aegypti genomes. The PolIII promoters were tested for their ability to express short-hairpin RNA (shRNA) targeted to firefly luciferase and to mediate RNA interference (RNAi) knockdown of a co-transfected luciferase reporter gene vector in AG-55 Anopheles gambiae and ATC-10 Aedes aegypti cells. Promoters capable of silencing expression of the co-transfected luciferase plasmid by up to 95% in AG-55 cells and up to 75% in ATC-10 cells were identified. RNase protection experiments allowed detection of the 19 nt luciferase short-interfering RNA (siRNA) in transfected cells. These findings indicate that mosquito U6snRNA gene promoters can be used for production of shRNA to induce the RNAi response in mosquito cells." @default.
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• W2129397265 date "2007-04-01" @default.
• W2129397265 modified "2023-10-16" @default.
• W2129397265 title "Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells" @default.
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• W2129397265 doi "https://doi.org/10.1111/j.1365-2583.2006.00714.x" @default.
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