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- W2129447335 abstract "Abstract During autogamy or conjugation in Paramecium, the micronucleus undergoes prezygotic and postzygotic nuclear divisions to result in new micro- and macronuclei, while the old macronucleus breaks down into fragments. The fragments appear to remain functional for some time, as evidenced by their ability to generate an intact macronucleus, via the process called macronuclear regeneration. On the other hand, macronuclear DNA degradation must take place, as suggested by a transfer of isotope-labeled material from the fragments to the developing macronuclear anlagen. We monitored electrophoretic profiles of DNA in autogamous and exautogamous cells of P. tetraurelia at 1-day intervals for 5 days, and in conjugating and exconjugant cells of P. multimicronucleatum treated with conjugation-inducing chemicals at 1–2 hr intervals for 2 days. We failed to detect DNA degradation in P. tetraurelia, but found a prominent DNA electrophoretic smear in starving exconjugant cells of P. multimicronucleatum 33–40 hr aft..." @default.
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- W2129447335 date "1998-12-01" @default.
- W2129447335 modified "2023-09-28" @default.
- W2129447335 title "Partial Autolysis of Macronuclear Fragments Revealed by Rapid DNA Degradation in Exconjugants of Paramecium multimicronucleatum" @default.
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- W2129447335 doi "https://doi.org/10.2108/zsj.15.849" @default.
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