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- W2129498229 abstract "Sixty-four lactococcal strains isolated from natural whey starters were screened for the presence of the nisin structural gene by polymerase chain reaction. Seven of them showed a specific PCR product of 320 bp; only two produced antagonistic activity and were resistant to nisin. Southern blots of SmaI-digested DNA from PCR-positive strains hybridized with a nisA probe displayed a location of the gene on different SmaI fragments. Among PCR-positive strains, nisin producers showed specific transcript after reverse transcriptase-PCR, as well as some non-nisin-producing strains. The RT-PCR product could not be shown in one non-nisin-producing PCR-positive strain." @default.
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- W2129498229 date "1996-11-01" @default.
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- W2129498229 title "Presence of non-functional nisin genes in<i>Lactococcus lactis</i>subsp.<i>lactis</i>isolated from natural starters" @default.
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- W2129498229 doi "https://doi.org/10.1111/j.1574-6968.1996.tb08552.x" @default.
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