FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129591311> ?p ?o ?g. }

• W2129591311 endingPage "3985" @default.
• W2129591311 startingPage "3977" @default.
• W2129591311 abstract "In rabbits and probably in man, GH-binding protein (GHBP) is generated from proteolysis of GH receptor (GHR). The present study describes the modulation of spontaneous release of GHBP into the culture medium in relation to cellular GH receptor (GHR) in Chinese hamster ovary cells transfected with rabbit GHR complementary DNA. Secretion of GHBP (approximately 50K protein) from these cells was dependent on time, percentage of FCS, temperature, and protein synthesis. GHBP was detected in the medium at 30 min, and a linear increase was observed over the next 4 h. GHBP release was reduced by low incubation temperature, suggesting that GHBP cleavage is an energy-requiring mechanism. N-Ethylmaleimide (500 microM for 30 min at 30 C) markedly increased GHBP secretion, matched by a corresponding decrease in GHR. However, the lack of effect of N-ethyl-maleimide observed at 4 C further confirms the temperature dependence of GHBP release. We have attempted to characterize the GHBP release protease with a number of recognized protease inhibitors. Benzamidine (10 mM) was the only protease inhibitor that reduced GHBP release; however, it also reduced the cellular GHR level. Cycloheximide (20 micrograms/ml) caused a parallel disappearance of cellular GHR and secreted GHBP with a half-life of about 50 min, but increased GHR messenger RNA expression (superinduction). Indeed, 4 h after removal of cycloheximide, GHR and GHBP were increased by 181% and 369%, respectively, compared to the control value. In summary, Chinese hamster ovary cells expressing rabbit GHR provide a useful cellular model system for studies on the mechanism of GHBP generation from GHR and its physiological importance." @default.
• W2129591311 created "2016-06-24" @default.
• W2129591311 creator A5000909905 @default.
• W2129591311 creator A5005030965 @default.
• W2129591311 creator A5034537671 @default.
• W2129591311 creator A5045828711 @default.
• W2129591311 creator A5053858269 @default.
• W2129591311 creator A5083534147 @default.
• W2129591311 date "1996-09-01" @default.
• W2129591311 modified "2023-10-17" @default.
• W2129591311 title "Regulation of cellular rabbit growth hormone (GH) receptor and GH-binding protein generation in vitro." @default.
• W2129591311 cites W141846371 @default.
• W2129591311 cites W1497106391 @default.
• W2129591311 cites W1503259740 @default.
• W2129591311 cites W1606315898 @default.
• W2129591311 cites W1775749144 @default.
• W2129591311 cites W1979938372 @default.
• W2129591311 cites W1983649348 @default.
• W2129591311 cites W1993776398 @default.
• W2129591311 cites W1997395577 @default.
• W2129591311 cites W1999875534 @default.
• W2129591311 cites W2001351034 @default.
• W2129591311 cites W2001575059 @default.
• W2129591311 cites W2002879545 @default.
• W2129591311 cites W2003604055 @default.
• W2129591311 cites W2011710244 @default.
• W2129591311 cites W2015736287 @default.
• W2129591311 cites W2016473857 @default.
• W2129591311 cites W2034296167 @default.
• W2129591311 cites W2042555886 @default.
• W2129591311 cites W2049732225 @default.
• W2129591311 cites W2053519440 @default.
• W2129591311 cites W2062686544 @default.
• W2129591311 cites W2071182563 @default.
• W2129591311 cites W2078932870 @default.
• W2129591311 cites W2086423720 @default.
• W2129591311 cites W2089728262 @default.
• W2129591311 cites W2108502920 @default.
• W2129591311 cites W2127356536 @default.
• W2129591311 cites W2137194103 @default.
• W2129591311 cites W2144294273 @default.
• W2129591311 cites W2170342180 @default.
• W2129591311 cites W2481702223 @default.
• W2129591311 cites W37545008 @default.
• W2129591311 doi "https://doi.org/10.1210/endo.137.9.8756574" @default.
• W2129591311 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8756574" @default.
• W2129591311 hasPublicationYear "1996" @default.
• W2129591311 type Work @default.
• W2129591311 sameAs 2129591311 @default.
• W2129591311 citedByCount "14" @default.
• W2129591311 crossrefType "journal-article" @default.
• W2129591311 hasAuthorship W2129591311A5000909905 @default.
• W2129591311 hasAuthorship W2129591311A5005030965 @default.
• W2129591311 hasAuthorship W2129591311A5034537671 @default.
• W2129591311 hasAuthorship W2129591311A5045828711 @default.
• W2129591311 hasAuthorship W2129591311A5053858269 @default.
• W2129591311 hasAuthorship W2129591311A5083534147 @default.
• W2129591311 hasBestOaLocation W21295913111 @default.
• W2129591311 hasConcept C104317684 @default.
• W2129591311 hasConcept C113675107 @default.
• W2129591311 hasConcept C126322002 @default.
• W2129591311 hasConcept C134018914 @default.
• W2129591311 hasConcept C153911025 @default.
• W2129591311 hasConcept C170493617 @default.
• W2129591311 hasConcept C175656101 @default.
• W2129591311 hasConcept C181199279 @default.
• W2129591311 hasConcept C2776714187 @default.
• W2129591311 hasConcept C2778128677 @default.
• W2129591311 hasConcept C2778328710 @default.
• W2129591311 hasConcept C2984496839 @default.
• W2129591311 hasConcept C3675279 @default.
• W2129591311 hasConcept C553089730 @default.
• W2129591311 hasConcept C55493867 @default.
• W2129591311 hasConcept C71315377 @default.
• W2129591311 hasConcept C71924100 @default.
• W2129591311 hasConcept C86803240 @default.
• W2129591311 hasConceptScore W2129591311C104317684 @default.
• W2129591311 hasConceptScore W2129591311C113675107 @default.
• W2129591311 hasConceptScore W2129591311C126322002 @default.
• W2129591311 hasConceptScore W2129591311C134018914 @default.
• W2129591311 hasConceptScore W2129591311C153911025 @default.
• W2129591311 hasConceptScore W2129591311C170493617 @default.
• W2129591311 hasConceptScore W2129591311C175656101 @default.
• W2129591311 hasConceptScore W2129591311C181199279 @default.
• W2129591311 hasConceptScore W2129591311C2776714187 @default.
• W2129591311 hasConceptScore W2129591311C2778128677 @default.
• W2129591311 hasConceptScore W2129591311C2778328710 @default.
• W2129591311 hasConceptScore W2129591311C2984496839 @default.
• W2129591311 hasConceptScore W2129591311C3675279 @default.
• W2129591311 hasConceptScore W2129591311C553089730 @default.
• W2129591311 hasConceptScore W2129591311C55493867 @default.
• W2129591311 hasConceptScore W2129591311C71315377 @default.
• W2129591311 hasConceptScore W2129591311C71924100 @default.
• W2129591311 hasConceptScore W2129591311C86803240 @default.
• W2129591311 hasIssue "9" @default.
• W2129591311 hasLocation W21295913111 @default.
• W2129591311 hasLocation W21295913112 @default.
• W2129591311 hasOpenAccess W2129591311 @default.

• next