Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129598977> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2129598977 endingPage "207" @default.
- W2129598977 startingPage "201" @default.
- W2129598977 abstract "Abstract Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) belong to the group of microbial polyesters. The key enzyme for mcl-PHA biosynthesis is type II PHA synthase. The gene phaC 2 encoding type II PHA synthase was placed under the control of psbA -pro and psbA -ter of rice ( Oryza sativa ) to construct a phaC 2 cassette, which was ligated with the screening marker gene aadA cassette ( prrn – aadA – TpsbA -ter). These recombined fragments were cloned between the plastid rbcL and accD genes for targeting to the large single copy region of the chloroplast genome. A chloroplast transformation vector, pTC2, was constructed and introduced into the tobacco ( Nicotiana tobacum ) chloroplast genome by particle bombardment. PCR and Southern blot analysis confirmed stable integration of phaC 2 into the chloroplast genomes of T 0 and T 1 transgenic plants, and T 1 transgenic plants exhibited homoplasmy. The expression of phaC 2 at transcription level was detected by reverse transcriptase–polymerase chain reaction (RT-PCR). Recombinant transgenes in the tobacco chloroplast genome were maternally inherited and were not transmitted via pollen when out-crossed with untransformed female plants. To our knowledge, this is the first report on the stable transformation of phaC 2 encoding type II PHA synthase in tobacco via chloroplast genetic engineering." @default.
- W2129598977 created "2016-06-24" @default.
- W2129598977 creator A5017130185 @default.
- W2129598977 creator A5018681710 @default.
- W2129598977 creator A5036692441 @default.
- W2129598977 creator A5049424355 @default.
- W2129598977 creator A5056716675 @default.
- W2129598977 creator A5056882178 @default.
- W2129598977 date "2006-12-01" @default.
- W2129598977 modified "2023-10-18" @default.
- W2129598977 title "Stable transformation ofphaC2 gene in tobacco chloroplast genome" @default.
- W2129598977 cites W1964933772 @default.
- W2129598977 cites W1974089913 @default.
- W2129598977 cites W1977537416 @default.
- W2129598977 cites W1978880365 @default.
- W2129598977 cites W1981085183 @default.
- W2129598977 cites W1984725193 @default.
- W2129598977 cites W1997105428 @default.
- W2129598977 cites W2006816471 @default.
- W2129598977 cites W2018822159 @default.
- W2129598977 cites W2029417091 @default.
- W2129598977 cites W2053167380 @default.
- W2129598977 cites W2060097978 @default.
- W2129598977 cites W207050664 @default.
- W2129598977 cites W2078182305 @default.
- W2129598977 cites W2086947027 @default.
- W2129598977 cites W2113674855 @default.
- W2129598977 cites W2115251455 @default.
- W2129598977 cites W2118988163 @default.
- W2129598977 cites W2170152151 @default.
- W2129598977 cites W2179908318 @default.
- W2129598977 cites W4211245217 @default.
- W2129598977 cites W4242557011 @default.
- W2129598977 cites W55419897 @default.
- W2129598977 doi "https://doi.org/10.1079/cjb2006109" @default.
- W2129598977 hasPublicationYear "2006" @default.
- W2129598977 type Work @default.
- W2129598977 sameAs 2129598977 @default.
- W2129598977 citedByCount "0" @default.
- W2129598977 crossrefType "journal-article" @default.
- W2129598977 hasAuthorship W2129598977A5017130185 @default.
- W2129598977 hasAuthorship W2129598977A5018681710 @default.
- W2129598977 hasAuthorship W2129598977A5036692441 @default.
- W2129598977 hasAuthorship W2129598977A5049424355 @default.
- W2129598977 hasAuthorship W2129598977A5056716675 @default.
- W2129598977 hasAuthorship W2129598977A5056882178 @default.
- W2129598977 hasConcept C104317684 @default.
- W2129598977 hasConcept C133307689 @default.
- W2129598977 hasConcept C141231307 @default.
- W2129598977 hasConcept C153911025 @default.
- W2129598977 hasConcept C204241405 @default.
- W2129598977 hasConcept C48050816 @default.
- W2129598977 hasConcept C49105822 @default.
- W2129598977 hasConcept C54355233 @default.
- W2129598977 hasConcept C69305403 @default.
- W2129598977 hasConcept C86803240 @default.
- W2129598977 hasConceptScore W2129598977C104317684 @default.
- W2129598977 hasConceptScore W2129598977C133307689 @default.
- W2129598977 hasConceptScore W2129598977C141231307 @default.
- W2129598977 hasConceptScore W2129598977C153911025 @default.
- W2129598977 hasConceptScore W2129598977C204241405 @default.
- W2129598977 hasConceptScore W2129598977C48050816 @default.
- W2129598977 hasConceptScore W2129598977C49105822 @default.
- W2129598977 hasConceptScore W2129598977C54355233 @default.
- W2129598977 hasConceptScore W2129598977C69305403 @default.
- W2129598977 hasConceptScore W2129598977C86803240 @default.
- W2129598977 hasIssue "3" @default.
- W2129598977 hasLocation W21295989771 @default.
- W2129598977 hasOpenAccess W2129598977 @default.
- W2129598977 hasPrimaryLocation W21295989771 @default.
- W2129598977 hasRelatedWork W1977765927 @default.
- W2129598977 hasRelatedWork W1998434418 @default.
- W2129598977 hasRelatedWork W2003674398 @default.
- W2129598977 hasRelatedWork W2100405084 @default.
- W2129598977 hasRelatedWork W2162452211 @default.
- W2129598977 hasRelatedWork W2325037381 @default.
- W2129598977 hasRelatedWork W2740943251 @default.
- W2129598977 hasRelatedWork W2753521576 @default.
- W2129598977 hasRelatedWork W39413609 @default.
- W2129598977 hasRelatedWork W2181488328 @default.
- W2129598977 hasVolume "3" @default.
- W2129598977 isParatext "false" @default.
- W2129598977 isRetracted "false" @default.
- W2129598977 magId "2129598977" @default.
- W2129598977 workType "article" @default.