Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129642645> ?p ?o ?g. }
- W2129642645 endingPage "R88" @default.
- W2129642645 startingPage "R88" @default.
- W2129642645 abstract "Gout is characterized by episodes of intense joint inflammation in response to intra-articular monosodium urate monohydrate (MSU) crystals. miR-155 is crucial for the proinflammatory activation of human myeloid cells and antigen-driven inflammatory arthritis. The functional role of miR-155 in acute gouty arthritis has not been defined. Therefore, the aim of this study was to examine the role of miR-155 in pathogenesis of acute gouty arthritis. Samples from 14 patients with acute gouty arthritis and 10 healthy controls (HCs) were obtained. Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) were cultured in vitro with MSU crystals, and gene expression (human miR-155 and SHIP-1) were assessed by real-time PCR. THP-1 cells were stimulated by MSU crystals and/or miR-155 transfection and then subjected to Western blot analysis. Levels of human tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-1β in cell culture supernatants were measured by Luminex. Immunohistochemistry was performed on formalin-fixed gout tissues with anti–SHIP-1 antibody. A C57BL/6 J male mouse model of gout was used to analyze the expressions of miR-155, SHIP-1, and inflammatory cytokines. The samples from gouty arthritis were highly enriched in miR-155, with levels of expression being higher than those found in PBMC from HC. Treatment of the cells with MSU crystals strongly induced miR-155. In addition, overexpression of miR-155 in the cells decreased levels of SHIP-1 and promoted production of MSU-induced proinflammatory cytokines, such as TNF-α and IL-1β. Consistent with in vitro observations, miR-155 expression was elevated in the mouse model of gout. The production of inflammatory cytokines was markedly increased in MSU crystal induced peritonitis mice. Overexpression of miR-155 in the gouty SFMC leads to suppress SHIP-1 levels and enhance proinflammatory cytokines." @default.
- W2129642645 created "2016-06-24" @default.
- W2129642645 creator A5006407937 @default.
- W2129642645 creator A5006915740 @default.
- W2129642645 creator A5007675211 @default.
- W2129642645 creator A5016455945 @default.
- W2129642645 creator A5016808511 @default.
- W2129642645 creator A5024843746 @default.
- W2129642645 creator A5029808309 @default.
- W2129642645 creator A5043724477 @default.
- W2129642645 creator A5047317750 @default.
- W2129642645 creator A5065859718 @default.
- W2129642645 creator A5067036969 @default.
- W2129642645 creator A5090003158 @default.
- W2129642645 date "2014-01-01" @default.
- W2129642645 modified "2023-10-07" @default.
- W2129642645 title "MicroRNA-155 as a proinflammatory regulator via SHIP-1 down-regulation in acute gouty arthritis" @default.
- W2129642645 cites W144423133 @default.
- W2129642645 cites W1529674879 @default.
- W2129642645 cites W1974102799 @default.
- W2129642645 cites W1979206745 @default.
- W2129642645 cites W1985806780 @default.
- W2129642645 cites W1988140132 @default.
- W2129642645 cites W1988201931 @default.
- W2129642645 cites W2009800174 @default.
- W2129642645 cites W2014869583 @default.
- W2129642645 cites W2016112105 @default.
- W2129642645 cites W2016207805 @default.
- W2129642645 cites W2024475404 @default.
- W2129642645 cites W2026622665 @default.
- W2129642645 cites W2036534625 @default.
- W2129642645 cites W2037678644 @default.
- W2129642645 cites W2045895870 @default.
- W2129642645 cites W2047321895 @default.
- W2129642645 cites W2048178760 @default.
- W2129642645 cites W2049772720 @default.
- W2129642645 cites W2059695215 @default.
- W2129642645 cites W2060830991 @default.
- W2129642645 cites W2079294041 @default.
- W2129642645 cites W2101125536 @default.
- W2129642645 cites W2102824849 @default.
- W2129642645 cites W2108014241 @default.
- W2129642645 cites W2127669794 @default.
- W2129642645 cites W2133571578 @default.
- W2129642645 cites W2158386218 @default.
- W2129642645 cites W2164113951 @default.
- W2129642645 cites W2171139781 @default.
- W2129642645 doi "https://doi.org/10.1186/ar4531" @default.
- W2129642645 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4060367" @default.
- W2129642645 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24708712" @default.
- W2129642645 hasPublicationYear "2014" @default.
- W2129642645 type Work @default.
- W2129642645 sameAs 2129642645 @default.
- W2129642645 citedByCount "73" @default.
- W2129642645 countsByYear W21296426452014 @default.
- W2129642645 countsByYear W21296426452015 @default.
- W2129642645 countsByYear W21296426452016 @default.
- W2129642645 countsByYear W21296426452017 @default.
- W2129642645 countsByYear W21296426452018 @default.
- W2129642645 countsByYear W21296426452019 @default.
- W2129642645 countsByYear W21296426452020 @default.
- W2129642645 countsByYear W21296426452021 @default.
- W2129642645 countsByYear W21296426452022 @default.
- W2129642645 countsByYear W21296426452023 @default.
- W2129642645 crossrefType "journal-article" @default.
- W2129642645 hasAuthorship W2129642645A5006407937 @default.
- W2129642645 hasAuthorship W2129642645A5006915740 @default.
- W2129642645 hasAuthorship W2129642645A5007675211 @default.
- W2129642645 hasAuthorship W2129642645A5016455945 @default.
- W2129642645 hasAuthorship W2129642645A5016808511 @default.
- W2129642645 hasAuthorship W2129642645A5024843746 @default.
- W2129642645 hasAuthorship W2129642645A5029808309 @default.
- W2129642645 hasAuthorship W2129642645A5043724477 @default.
- W2129642645 hasAuthorship W2129642645A5047317750 @default.
- W2129642645 hasAuthorship W2129642645A5065859718 @default.
- W2129642645 hasAuthorship W2129642645A5067036969 @default.
- W2129642645 hasAuthorship W2129642645A5090003158 @default.
- W2129642645 hasBestOaLocation W21296426451 @default.
- W2129642645 hasConcept C104317684 @default.
- W2129642645 hasConcept C126322002 @default.
- W2129642645 hasConcept C137061746 @default.
- W2129642645 hasConcept C142724271 @default.
- W2129642645 hasConcept C145059251 @default.
- W2129642645 hasConcept C164027704 @default.
- W2129642645 hasConcept C17991360 @default.
- W2129642645 hasConcept C198451711 @default.
- W2129642645 hasConcept C202751555 @default.
- W2129642645 hasConcept C203014093 @default.
- W2129642645 hasConcept C204787440 @default.
- W2129642645 hasConcept C2776164576 @default.
- W2129642645 hasConcept C2776268809 @default.
- W2129642645 hasConcept C2776821229 @default.
- W2129642645 hasConcept C2776914184 @default.
- W2129642645 hasConcept C2777077863 @default.
- W2129642645 hasConcept C2778455985 @default.
- W2129642645 hasConcept C2780402116 @default.
- W2129642645 hasConcept C55493867 @default.
- W2129642645 hasConcept C71924100 @default.