FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129695830> ?p ?o ?g. }

• W2129695830 endingPage "H785" @default.
• W2129695830 startingPage "H776" @default.
• W2129695830 abstract "Suppressor of cytokine signaling-3 (SOCS3) is an intracellular negative regulator of cytokine signaling pathway. We recently found significant reduction in SOCS3 expression in coronary artery smooth muscle cells (CASMCs) of atherosclerotic swine and also in vitro cultured cells. Here, we investigated the underlying mechanisms of SOCS3 downregulation by IGF-1 and TNF-α in human CASMCs(hCASMCs). We propose that hypermethylation of CpG islands in the SOCS3 promoter is responsible for decrease in SOCS3 expression involving STAT3 and NFkB-p65 interaction. Western blot and qPCR data revealed significant upregulation of SOCS3 (6- to 10-fold) in hCASMC when treated individually with TNF-α (100 ng/ml) or IGF-1 (100 ng/ml). However, a significant decrease (5-fold) was observed by the combined treatment with TNF-α and IGF-1 compared with individual stimulation. IGF-1 phosphorylated STAT3 and TNF-α-activated NF-κB in hCASMCs. In the nuclear extract of hCASMCs stimulated with both TNF-α and IGF-1, there was an interaction between NF-κB-p65 and pSTAT3, as determined by co-immunoprecipitation. Knockdown of STAT3 by small interfering RNA abolished SOCS3 expression in response to IGF-1. Methylation-specific PCR confirmed hypermethylation of SOCS3 promoter in hCASMCs stimulated with both TNF-α and IGF-1, and this was positively associated with elevated levels of DNA methyltransferase-I (9- to 10-fold). Knockdown of DNMT1 increased SOCS3 expression in IGF-1+TNF-α-stimulated cells. Downregulation of SOCS3 in the presence of both TNF-α and IGF-1 in hCASMCs is due to SOCS3 promoter hypermethylation involving STAT3-NFkBp65 interaction. Because TNF-α and IGF-1 are released due to mechanical injury during coronary intervention, hypermethylation of SOCS3 gene could be an underlying mechanism of intimal hyperplasia and restenosis." @default.
• W2129695830 created "2016-06-24" @default.
• W2129695830 creator A5000800160 @default.
• W2129695830 creator A5005088993 @default.
• W2129695830 creator A5006699471 @default.
• W2129695830 creator A5019762392 @default.
• W2129695830 date "2013-03-15" @default.
• W2129695830 modified "2023-10-02" @default.
• W2129695830 title "SOCS3 promotor hypermethylation and STAT3-NF-κB interaction downregulate SOCS3 expression in human coronary artery smooth muscle cells" @default.
• W2129695830 cites W1593893099 @default.
• W2129695830 cites W1616387479 @default.
• W2129695830 cites W1638480194 @default.
• W2129695830 cites W1888783223 @default.
• W2129695830 cites W1965464733 @default.
• W2129695830 cites W1969218281 @default.
• W2129695830 cites W1970308473 @default.
• W2129695830 cites W1972699527 @default.
• W2129695830 cites W1979299941 @default.
• W2129695830 cites W1979337039 @default.
• W2129695830 cites W1980153644 @default.
• W2129695830 cites W1981745443 @default.
• W2129695830 cites W1982078599 @default.
• W2129695830 cites W1983667136 @default.
• W2129695830 cites W2000974116 @default.
• W2129695830 cites W2001158436 @default.
• W2129695830 cites W2008295144 @default.
• W2129695830 cites W2014420892 @default.
• W2129695830 cites W2023561138 @default.
• W2129695830 cites W2029169197 @default.
• W2129695830 cites W2040257851 @default.
• W2129695830 cites W2047972161 @default.
• W2129695830 cites W2051680428 @default.
• W2129695830 cites W2059287377 @default.
• W2129695830 cites W2060339708 @default.
• W2129695830 cites W2060402899 @default.
• W2129695830 cites W2069498567 @default.
• W2129695830 cites W2071985572 @default.
• W2129695830 cites W2079422313 @default.
• W2129695830 cites W2081107312 @default.
• W2129695830 cites W2082627972 @default.
• W2129695830 cites W2086297948 @default.
• W2129695830 cites W2086840459 @default.
• W2129695830 cites W2089889294 @default.
• W2129695830 cites W2093188241 @default.
• W2129695830 cites W2103422355 @default.
• W2129695830 cites W2108486371 @default.
• W2129695830 cites W2111379723 @default.
• W2129695830 cites W2112653342 @default.
• W2129695830 cites W2119733834 @default.
• W2129695830 cites W2119906430 @default.
• W2129695830 cites W2143001254 @default.
• W2129695830 cites W2149192856 @default.
• W2129695830 cites W2152000177 @default.
• W2129695830 cites W2156387051 @default.
• W2129695830 cites W2164967233 @default.
• W2129695830 cites W2168692939 @default.
• W2129695830 cites W4313347996 @default.
• W2129695830 cites W63837087 @default.
• W2129695830 doi "https://doi.org/10.1152/ajpheart.00570.2012" @default.
• W2129695830 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3602771" @default.
• W2129695830 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23335796" @default.
• W2129695830 hasPublicationYear "2013" @default.
• W2129695830 type Work @default.
• W2129695830 sameAs 2129695830 @default.
• W2129695830 citedByCount "43" @default.
• W2129695830 countsByYear W21296958302014 @default.
• W2129695830 countsByYear W21296958302015 @default.
• W2129695830 countsByYear W21296958302016 @default.
• W2129695830 countsByYear W21296958302017 @default.
• W2129695830 countsByYear W21296958302018 @default.
• W2129695830 countsByYear W21296958302019 @default.
• W2129695830 countsByYear W21296958302020 @default.
• W2129695830 countsByYear W21296958302021 @default.
• W2129695830 countsByYear W21296958302022 @default.
• W2129695830 countsByYear W21296958302023 @default.
• W2129695830 crossrefType "journal-article" @default.
• W2129695830 hasAuthorship W2129695830A5000800160 @default.
• W2129695830 hasAuthorship W2129695830A5005088993 @default.
• W2129695830 hasAuthorship W2129695830A5006699471 @default.
• W2129695830 hasAuthorship W2129695830A5019762392 @default.
• W2129695830 hasBestOaLocation W21296958302 @default.
• W2129695830 hasConcept C104317684 @default.
• W2129695830 hasConcept C126042315 @default.
• W2129695830 hasConcept C127561419 @default.
• W2129695830 hasConcept C150194340 @default.
• W2129695830 hasConcept C153911025 @default.
• W2129695830 hasConcept C173396325 @default.
• W2129695830 hasConcept C175211621 @default.
• W2129695830 hasConcept C185592680 @default.
• W2129695830 hasConcept C190727270 @default.
• W2129695830 hasConcept C203014093 @default.
• W2129695830 hasConcept C22615655 @default.
• W2129695830 hasConcept C2778690821 @default.
• W2129695830 hasConcept C2778923194 @default.
• W2129695830 hasConcept C33288867 @default.
• W2129695830 hasConcept C502942594 @default.
• W2129695830 hasConcept C54009773 @default.
• W2129695830 hasConcept C55493867 @default.

• next