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- W2129708844 abstract "of papers 33 3. Abstract of papers Paper I. Immune response of Atlantic salmon to recombinant flagellin Many viral vaccines used in aquaculture are unable to stimulate an appropriate level of immunity to withstand infection. By targeting specific components of the immune system it may be possible to trigger stronger, more effective responses to antigens. Flagellin has the ability to stimulate both the soluble and membrane-bound forms of toll-like receptor 5 (TLR5) in salmon leading to a proinflammatory response and activation of both the innate and adaptive immune system. In this study flagellin (FlaD from Vibrio anguillarum) was recombinantly produced in two forms, full-length (FDL) and a truncated form (FDS) with portions of the Nand C-termini removed to prevent polymerization. FDS was used to produce an antibody that was able to bind both forms of flagellin in immunoblot analysis. In cell culture using COS-7 cells, FDL was shown to stimulate the NFB pathway more effectively than FDS. Both forms of flagellin were used as an adjuvant with the antigen LPH (Hemocyanin from Limulus polyphemus hemolymph) in an immunization dose response study. FDS and FDL stimulated the innate immune system of salmon inducing proinflammatory -6, IL-8, and IL-regulated (p<0.05) in the spleen. TLR5S was more highly up-regulated than TLR5M indicating that the soluble form of TLR5 may play an important role in the innate immune response in salmon. ELISA analysis showed that the use of flagellin as an adjuvant with LPH was not able to significantly induce flagellin or LPH antibodies. This study shows that flagellin has the potential to be a highly effective adjuvant for salmon immunization, but further research is needed." @default.
- W2129708844 created "2016-06-24" @default.
- W2129708844 creator A5070628545 @default.
- W2129708844 date "2011-10-18" @default.
- W2129708844 modified "2023-09-26" @default.
- W2129708844 title "Bacterial flagellin- a novel adjuvant for vaccine strategies" @default.
- W2129708844 cites W1483162601 @default.
- W2129708844 cites W1491252070 @default.
- W2129708844 cites W1498138425 @default.
- W2129708844 cites W1505229526 @default.
- W2129708844 cites W1508362664 @default.
- W2129708844 cites W1519633296 @default.
- W2129708844 cites W1524159069 @default.
- W2129708844 cites W1534016046 @default.
- W2129708844 cites W1545047818 @default.
- W2129708844 cites W1561224282 @default.
- W2129708844 cites W1570790762 @default.
- W2129708844 cites W1581620735 @default.
- W2129708844 cites W158312939 @default.
- W2129708844 cites W1664413187 @default.
- W2129708844 cites W1828093076 @default.
- W2129708844 cites W1963223912 @default.
- W2129708844 cites W1963736364 @default.
- W2129708844 cites W1966892322 @default.
- W2129708844 cites W1968087807 @default.
- W2129708844 cites W1968693613 @default.
- W2129708844 cites W1969022040 @default.
- W2129708844 cites W1971902835 @default.
- W2129708844 cites W1972148325 @default.
- W2129708844 cites W1972908383 @default.
- W2129708844 cites W1973101085 @default.
- W2129708844 cites W1973504815 @default.
- W2129708844 cites W1973705388 @default.
- W2129708844 cites W1975200469 @default.
- W2129708844 cites W1975828419 @default.
- W2129708844 cites W1976028300 @default.
- W2129708844 cites W1976927528 @default.
- W2129708844 cites W1978926805 @default.
- W2129708844 cites W1979132095 @default.
- W2129708844 cites W1980109413 @default.
- W2129708844 cites W1981400681 @default.
- W2129708844 cites W1982078047 @default.
- W2129708844 cites W1982806555 @default.
- W2129708844 cites W1983966300 @default.
- W2129708844 cites W1984420944 @default.
- W2129708844 cites W1985627935 @default.
- W2129708844 cites W1985973479 @default.
- W2129708844 cites W1986795056 @default.
- W2129708844 cites W1987535382 @default.
- W2129708844 cites W1990177413 @default.
- W2129708844 cites W1990580731 @default.
- W2129708844 cites W1991024712 @default.
- W2129708844 cites W1993013835 @default.
- W2129708844 cites W1993952505 @default.
- W2129708844 cites W1995296281 @default.
- W2129708844 cites W1996050664 @default.
- W2129708844 cites W1996670911 @default.
- W2129708844 cites W1997082390 @default.
- W2129708844 cites W2000890891 @default.
- W2129708844 cites W2001178191 @default.
- W2129708844 cites W2001747259 @default.
- W2129708844 cites W2003405010 @default.
- W2129708844 cites W2003785366 @default.
- W2129708844 cites W2004773897 @default.
- W2129708844 cites W2005573508 @default.
- W2129708844 cites W2006246742 @default.
- W2129708844 cites W2007041007 @default.
- W2129708844 cites W2009651579 @default.
- W2129708844 cites W2009874664 @default.
- W2129708844 cites W2011302465 @default.
- W2129708844 cites W2013089263 @default.
- W2129708844 cites W2014227845 @default.
- W2129708844 cites W2014552502 @default.
- W2129708844 cites W2014972951 @default.
- W2129708844 cites W2017155427 @default.
- W2129708844 cites W2017176041 @default.
- W2129708844 cites W2021487333 @default.
- W2129708844 cites W2022023427 @default.
- W2129708844 cites W2022638359 @default.
- W2129708844 cites W2022803237 @default.
- W2129708844 cites W2023483341 @default.
- W2129708844 cites W2024089410 @default.
- W2129708844 cites W2024238985 @default.
- W2129708844 cites W2024961499 @default.
- W2129708844 cites W2025795405 @default.
- W2129708844 cites W2027983878 @default.
- W2129708844 cites W2028100864 @default.
- W2129708844 cites W2028379781 @default.
- W2129708844 cites W2028584099 @default.
- W2129708844 cites W2030422301 @default.
- W2129708844 cites W2031334014 @default.
- W2129708844 cites W2031671468 @default.
- W2129708844 cites W2033560950 @default.
- W2129708844 cites W2033927088 @default.
- W2129708844 cites W2034087823 @default.
- W2129708844 cites W2034265657 @default.
- W2129708844 cites W2034722126 @default.
- W2129708844 cites W2035124958 @default.
- W2129708844 cites W2035380145 @default.
- W2129708844 cites W2037368492 @default.