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- W2129741625 abstract "We earlier described an apparatus and optical method that permit quantitative measurement of the adsorption of a solute to a gas-liquid interface [K.C. Hoang, D. Malakhov, W.E. Momsen and H.L. Brockman (2006) Anal. Chem. 78, 1657-1664]. The interface may or may not support a protein, lipid or other monolayer. A potential limitation to such a flow cell, being probed optically through the monolayer, is concomitant solute adsorption to the solid trough bottom, which is parallel to but displaced from the monolayer being studied. To circumvent this unwanted adsorption we introduced a flowing sucrose solution under the flowing solute solution that supports the monolayer. In this way solute is prevented from reaching the trough bottom and any solute which might adsorb to or diffuse through the sucrose-solute solution interface is rapidly moved from the optical path by flow to the trough drain. Eliminating the need to chemically modify the trough bottom by introducing a sucrose layer allows trough dimensions to be reduced, allows robust and fully automated cleaning between experiments and improves reproducibility of solute adsorption measurements. An additional feature of the new configuration is control of the thickness of the solute solution layer, which conserves reagents and enhances the sensitivity of solute adsorption measurements. The apparatus was used to characterize the interaction of a fluorescent protein antigen with an antibody monolayer and to the initial rate of adsorption of bovine serum albumin to phospholipid-diacylglycerol monolayers of differing lipid compositions but approximately constant surface pressure. Supported by USPHS HL-49180 and the Hormel Foundation." @default.
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- W2129741625 date "2011-02-01" @default.
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- W2129741625 title "An Improved Open Microfluidic Flow Cell for Measuring Solute Adsorption to Monolayers" @default.
- W2129741625 doi "https://doi.org/10.1016/j.bpj.2010.12.2984" @default.
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