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W2129789036 abstract "Nine sows were monitored for leukocyte subsets in mammary secretions during lactation. Using flow cytometry, percentages of monocytes/macrophages (CD45 + CD14 + ), T cells (CD3 + ), and their subpopulations (CD4 + , CD8 + and double positive (DP) CD4 + CD8 + ), and B cells (CD21 + ) in colostrum and milk were determined. The mean percentage of CD3 + cells was relatively stable throughout lactation. About 40% of colostral lymphocytes were identified as CD3 + , while in milk they comprised from 24% to 39%. The percentage of both CD8 + and CD4 + cells were relatively stable during lactation, beside significant decrease at the last day of lactation. Double positive cells comprised a small percentage of cells and did not exceed 7.5%. The frequency of DP lymphocytes increased significantly 6 h after parturition, and then remained relatively stable until 20 th d of lactation, followed by a significant decrease at day 28 postpartum. The CD8 + cells prevailed over CD4 + cells throughout whole lactation. The CD4/CD8 ratio was similar during lactation period and ranged from 0.50 to 0.53 at the beginning and the end of lactation, respectively. Lymphocytes B represented about 20% of all lymphocytes of mammary secretions. CD45 + CD14 + cells ranged between 5.01 and 10.14 % of all leukocytes." @default.
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W2129789036 date "2010-01-01" @default.
W2129789036 modified "2023-09-23" @default.
W2129789036 title "FLOW CYTOMETRIC ANALYSIS OF LEUKOCYTES IN PORCINE MAMMARY SECRETIONS" @default.
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