FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2129920504> ?p ?o ?g. }

• W2129920504 endingPage "1737" @default.
• W2129920504 startingPage "1721" @default.
• W2129920504 abstract "Telomerase is an RNA-dependent reverse transcriptase that maintains telomeric DNA at a species-specific equilibrium length. To determine an upper limit for the number of telomerase molecules in a Saccharomyces cerevisiae cell, we have established real-time RT-PCR assays to quantify the noncoding telomerase RNA, TLC1. We find that the number of TLC1 molecules in a haploid yeast cell is ∼29, less than the number of chromosome ends (64) in late S-phase. Wild-type diploid cells contain ∼37 telomerase RNAs, while diploids heterozygous for a null tlc1 allele have half the wild-type amount, ∼19 TLC1 molecules. For comparison, there are ∼480 molecules of the U2 snRNA per haploid cell. We show that a biological consequence of this low level of telomerase is haploinsufficiency: A TLC1/tlc1Δ heterozygote maintains shorter telomeres. A dominant-negative telomerase RNA, with a deletion of the template for telomeric DNA synthesis, further demonstrates that yeast telomere length is sensitive to telomerase dosage. Sixfold overexpression of tlc1Δtemplate establishes a new telomere length set point, ∼160 bp shorter than wild type. Removing telomerase protein-interaction sites from the tlc1Δtemplate RNA mitigates the dominant-negative effect, suggesting that the tlc1Δtemplate RNA competes with wild-type TLC1 for a limited supply of telomerase proteins or for telomeres. Because yeast telomerase is tethered at chromosome ends, the finding that it may be outnumbered by its telomeric DNA substrates provides a new perspective for interpreting the results of telomere maintenance studies." @default.
• W2129920504 created "2016-06-24" @default.
• W2129920504 creator A5017573996 @default.
• W2129920504 creator A5047846104 @default.
• W2129920504 date "2006-08-07" @default.
• W2129920504 modified "2023-10-02" @default.
• W2129920504 title "Low abundance of telomerase in yeast: Implications for telomerase haploinsufficiency" @default.
• W2129920504 cites W1514461091 @default.
• W2129920504 cites W1520911672 @default.
• W2129920504 cites W1559760142 @default.
• W2129920504 cites W1606954381 @default.
• W2129920504 cites W1927581912 @default.
• W2129920504 cites W1948628402 @default.
• W2129920504 cites W1965035358 @default.
• W2129920504 cites W1966072209 @default.
• W2129920504 cites W1977129262 @default.
• W2129920504 cites W1977780474 @default.
• W2129920504 cites W1984280207 @default.
• W2129920504 cites W1985623818 @default.
• W2129920504 cites W1987765421 @default.
• W2129920504 cites W1995925983 @default.
• W2129920504 cites W1998405157 @default.
• W2129920504 cites W2000971601 @default.
• W2129920504 cites W2004561527 @default.
• W2129920504 cites W2008225233 @default.
• W2129920504 cites W2008814693 @default.
• W2129920504 cites W2010070807 @default.
• W2129920504 cites W2013947447 @default.
• W2129920504 cites W2017235322 @default.
• W2129920504 cites W2022920512 @default.
• W2129920504 cites W2024153222 @default.
• W2129920504 cites W2025843494 @default.
• W2129920504 cites W2026856852 @default.
• W2129920504 cites W2031936092 @default.
• W2129920504 cites W2032149581 @default.
• W2129920504 cites W2032349285 @default.
• W2129920504 cites W2032486291 @default.
• W2129920504 cites W2040204581 @default.
• W2129920504 cites W2041771646 @default.
• W2129920504 cites W2044485780 @default.
• W2129920504 cites W2045702252 @default.
• W2129920504 cites W2052066518 @default.
• W2129920504 cites W2056717473 @default.
• W2129920504 cites W2065841924 @default.
• W2129920504 cites W2084017895 @default.
• W2129920504 cites W2087339962 @default.
• W2129920504 cites W2102971989 @default.
• W2129920504 cites W2107665329 @default.
• W2129920504 cites W2109768043 @default.
• W2129920504 cites W2113168077 @default.
• W2129920504 cites W2114551234 @default.
• W2129920504 cites W2122740168 @default.
• W2129920504 cites W2125194621 @default.
• W2129920504 cites W2128132451 @default.
• W2129920504 cites W2138498743 @default.
• W2129920504 cites W2140944222 @default.
• W2129920504 cites W2140948740 @default.
• W2129920504 cites W2144417959 @default.
• W2129920504 cites W2146503498 @default.
• W2129920504 cites W2149641002 @default.
• W2129920504 cites W2151439223 @default.
• W2129920504 cites W2153321182 @default.
• W2129920504 cites W2156315323 @default.
• W2129920504 cites W2166024667 @default.
• W2129920504 cites W2167304272 @default.
• W2129920504 cites W2175722093 @default.
• W2129920504 cites W2886161256 @default.
• W2129920504 cites W4213265720 @default.
• W2129920504 cites W4239029175 @default.
• W2129920504 doi "https://doi.org/10.1261/rna.134706" @default.
• W2129920504 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1557690" @default.
• W2129920504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16894218" @default.
• W2129920504 hasPublicationYear "2006" @default.
• W2129920504 type Work @default.
• W2129920504 sameAs 2129920504 @default.
• W2129920504 citedByCount "113" @default.
• W2129920504 countsByYear W21299205042012 @default.
• W2129920504 countsByYear W21299205042013 @default.
• W2129920504 countsByYear W21299205042014 @default.
• W2129920504 countsByYear W21299205042015 @default.
• W2129920504 countsByYear W21299205042016 @default.
• W2129920504 countsByYear W21299205042017 @default.
• W2129920504 countsByYear W21299205042018 @default.
• W2129920504 countsByYear W21299205042019 @default.
• W2129920504 countsByYear W21299205042020 @default.
• W2129920504 countsByYear W21299205042021 @default.
• W2129920504 countsByYear W21299205042022 @default.
• W2129920504 countsByYear W21299205042023 @default.
• W2129920504 crossrefType "journal-article" @default.
• W2129920504 hasAuthorship W2129920504A5017573996 @default.
• W2129920504 hasAuthorship W2129920504A5047846104 @default.
• W2129920504 hasBestOaLocation W21299205041 @default.
• W2129920504 hasConcept C104317684 @default.
• W2129920504 hasConcept C125593758 @default.
• W2129920504 hasConcept C127716648 @default.
• W2129920504 hasConcept C153911025 @default.
• W2129920504 hasConcept C177336024 @default.
• W2129920504 hasConcept C34558173 @default.

• next