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• W2130151903 endingPage "2332" @default.
• W2130151903 startingPage "2315" @default.
• W2130151903 abstract "Micrococcal nuclease digestion of Dictyostelium discoideum nuclei from various developmental stages was used to investigate transcription-related changes in the chromatin structure of the coding region of four genes. Gene activity was determined by Northern blotting and nuclear run on experiments. During strong transcription of the developmentally regulated cysteine proteinase I gene, a smear superimposed on a nucleosomal ladder was observed, indicating perturbation of nucleosomal structure was occurring. However, two other developmentally regulated genes, discoidin I and pSC253, showed only slight nucleosome disruption during high levels of transcription. The chromatin structure of a fourth gene (pCZ22) was disrupted throughout development, even at those stages where transcription was greatly reduced. We suggest that although nucleosome structure can be transiently perturbed by the passage of the transcription complex in vivo, the degree of perturbation and the speed with which nucleosomes reassemble is also influenced by the DNA sequence." @default.
• W2130151903 created "2016-06-24" @default.
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• W2130151903 creator A5087918957 @default.
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• W2130151903 date "1989-01-01" @default.
• W2130151903 modified "2023-09-24" @default.
• W2130151903 title "The effects of transcription on the nucleosome structure of four<i>Dictyostelium</i>genes" @default.
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• W2130151903 doi "https://doi.org/10.1093/nar/17.6.2315" @default.
• W2130151903 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/317598" @default.
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• W2130151903 hasPublicationYear "1989" @default.
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