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- W2130201966 abstract "A bifunctional xylosidase/arabinofuranosidase gene (PcXyl) was cloned from the cDNA library of Phanerochaete chrysosporium and further expressed in Pichia pastoris. Enzymatic assay indicated that P. pastoris produced rPcXyl at a level of 26,141 U l−1. The xylosidase and arabinofuranosidase activities of rPcXyl were maximized, respectively, at pHs of 5.0 and 5.5 and temperatures of 45°C and 50°C. SDS-PAGE revealed a single band of purified rPcXyl of 83 kDa. Cu2+ and Zn2+ completely inhibited the enzyme activity of rPcXyl. The enzyme activity of rPcXyl was increased 151%, 126% and 123%, respectively, in the presence of glucose, xylose and arabinose at concentrations of 5 mM. rPcXyl hydrolyzed xylobiose to xylose and xylotriose to xylose and xylobiose, indicating rPcXyl acts as an exo-type enzyme. Additionally, rPcXyl enhanced xylose release from xylan substrates in synergy with rPcXynC." @default.
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- W2130201966 date "2013-08-01" @default.
- W2130201966 modified "2023-09-25" @default.
- W2130201966 title "Characterization of a recombinant bifunctional xylosidase/arabinofuranosidase from Phanerochaete chrysosporium" @default.
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- W2130201966 doi "https://doi.org/10.1016/j.jbiosc.2013.02.004" @default.
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