FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2130256319> ?p ?o ?g. }

• W2130256319 endingPage "11774" @default.
• W2130256319 startingPage "11767" @default.
• W2130256319 abstract "ABSTRACT Human metapneumovirus (hMPV) is an important cause of lower respiratory tract disease, particularly in infants and young children. hMPV has two major glycoproteins, G and F, which are responsible for virus attachment and membrane fusion, respectively. We investigated the role of cellular glycosaminoglycans (GAGs) and G protein in hMPV infection. The pretreatment of hMPV with soluble heparin markedly inhibited the infection of HEp-2 cells. Recombinant G protein, comprising the extracellular domain of G, bound to heparin-agarose columns and also to HEp-2 cells. hMPV infection and G protein binding to HEp-2 cells was inhibited by other soluble GAGs, including chondroitin sulfates, by the enzymatic removal of cell surface GAGs with GAG lyases or by the pretreatment of cells with basic fibroblast growth factor. The role of cellular GAGs was confirmed by the binding of G protein to wild-type CHO cells but not to GAG-deficient CHO-pgsA745 cells. An analysis of the G protein sequence revealed two adjacent clusters of positively charged amino acids ( 149 EKKKTRA 155 and 159 QRRGKGKE 166 ). Truncated G fragments were expressed, and only the fragment containing these putative heparin binding domains retained heparin binding. The alanine mutagenesis of charged residues in either of these regions resulted in the loss of binding to heparin and to HEp-2 cells, suggesting that both sites are likely to be required for hMPV attachment. These results, taken together with the inhibition of hMPV infection by soluble G protein, indicate an important role for G protein and cellular GAGs in hMPV infection." @default.
• W2130256319 created "2016-06-24" @default.
• W2130256319 creator A5003385363 @default.
• W2130256319 creator A5040961140 @default.
• W2130256319 creator A5052323829 @default.
• W2130256319 creator A5053332907 @default.
• W2130256319 date "2008-12-01" @default.
• W2130256319 modified "2023-09-26" @default.
• W2130256319 title "Role of Cellular Glycosaminoglycans and Charged Regions of Viral G Protein in Human Metapneumovirus Infection" @default.
• W2130256319 cites W1559464451 @default.
• W2130256319 cites W1596750278 @default.
• W2130256319 cites W1963854906 @default.
• W2130256319 cites W1972873370 @default.
• W2130256319 cites W1973993324 @default.
• W2130256319 cites W1999756472 @default.
• W2130256319 cites W2010817652 @default.
• W2130256319 cites W2011095598 @default.
• W2130256319 cites W2018133348 @default.
• W2130256319 cites W2025510141 @default.
• W2130256319 cites W2029394530 @default.
• W2130256319 cites W2030577907 @default.
• W2130256319 cites W2058201868 @default.
• W2130256319 cites W2068567885 @default.
• W2130256319 cites W2069094887 @default.
• W2130256319 cites W2080182849 @default.
• W2130256319 cites W2081356514 @default.
• W2130256319 cites W2091677322 @default.
• W2130256319 cites W2092751827 @default.
• W2130256319 cites W2102146833 @default.
• W2130256319 cites W2105375720 @default.
• W2130256319 cites W2105952979 @default.
• W2130256319 cites W2110198546 @default.
• W2130256319 cites W2125818108 @default.
• W2130256319 cites W2128289360 @default.
• W2130256319 cites W2133364492 @default.
• W2130256319 cites W2135180688 @default.
• W2130256319 cites W2137682982 @default.
• W2130256319 cites W2151282930 @default.
• W2130256319 cites W2152552492 @default.
• W2130256319 cites W2152854377 @default.
• W2130256319 cites W2159334094 @default.
• W2130256319 cites W2161233359 @default.
• W2130256319 cites W2170881661 @default.
• W2130256319 cites W2171602693 @default.
• W2130256319 cites W2171917679 @default.
• W2130256319 cites W2413594285 @default.
• W2130256319 doi "https://doi.org/10.1128/jvi.01208-08" @default.
• W2130256319 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2583676" @default.
• W2130256319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18786997" @default.
• W2130256319 hasPublicationYear "2008" @default.
• W2130256319 type Work @default.
• W2130256319 sameAs 2130256319 @default.
• W2130256319 citedByCount "63" @default.
• W2130256319 countsByYear W21302563192012 @default.
• W2130256319 countsByYear W21302563192013 @default.
• W2130256319 countsByYear W21302563192014 @default.
• W2130256319 countsByYear W21302563192015 @default.
• W2130256319 countsByYear W21302563192016 @default.
• W2130256319 countsByYear W21302563192017 @default.
• W2130256319 countsByYear W21302563192018 @default.
• W2130256319 countsByYear W21302563192019 @default.
• W2130256319 countsByYear W21302563192020 @default.
• W2130256319 countsByYear W21302563192021 @default.
• W2130256319 countsByYear W21302563192022 @default.
• W2130256319 countsByYear W21302563192023 @default.
• W2130256319 crossrefType "journal-article" @default.
• W2130256319 hasAuthorship W2130256319A5003385363 @default.
• W2130256319 hasAuthorship W2130256319A5040961140 @default.
• W2130256319 hasAuthorship W2130256319A5052323829 @default.
• W2130256319 hasAuthorship W2130256319A5053332907 @default.
• W2130256319 hasBestOaLocation W21302563191 @default.
• W2130256319 hasConcept C105702510 @default.
• W2130256319 hasConcept C108625454 @default.
• W2130256319 hasConcept C153074725 @default.
• W2130256319 hasConcept C153911025 @default.
• W2130256319 hasConcept C2776012195 @default.
• W2130256319 hasConcept C2778041096 @default.
• W2130256319 hasConcept C2780475896 @default.
• W2130256319 hasConcept C534529494 @default.
• W2130256319 hasConcept C54355233 @default.
• W2130256319 hasConcept C55493867 @default.
• W2130256319 hasConcept C81885089 @default.
• W2130256319 hasConcept C86803240 @default.
• W2130256319 hasConceptScore W2130256319C105702510 @default.
• W2130256319 hasConceptScore W2130256319C108625454 @default.
• W2130256319 hasConceptScore W2130256319C153074725 @default.
• W2130256319 hasConceptScore W2130256319C153911025 @default.
• W2130256319 hasConceptScore W2130256319C2776012195 @default.
• W2130256319 hasConceptScore W2130256319C2778041096 @default.
• W2130256319 hasConceptScore W2130256319C2780475896 @default.
• W2130256319 hasConceptScore W2130256319C534529494 @default.
• W2130256319 hasConceptScore W2130256319C54355233 @default.
• W2130256319 hasConceptScore W2130256319C55493867 @default.
• W2130256319 hasConceptScore W2130256319C81885089 @default.
• W2130256319 hasConceptScore W2130256319C86803240 @default.
• W2130256319 hasIssue "23" @default.
• W2130256319 hasLocation W21302563191 @default.
• W2130256319 hasLocation W21302563192 @default.

• next