Matches in SemOpenAlex for { <https://semopenalex.org/work/W2130398470> ?p ?o ?g. }
- W2130398470 endingPage "66" @default.
- W2130398470 startingPage "59" @default.
- W2130398470 abstract "When panned with a transient heat denaturation approach against target enzymes, a human V(H) (antibody heavy chain variable domain) phage display library yielded V(H)s with composite characteristics of binding, non-aggregation and reversible thermal unfolding. Moreover, selection was characterized by enrichment for V(H)s with (i) an even number of disulfide forming Cys residues in complementarity-determining region (CDR) 1 and CDR3 and (ii) acidic isoelectric points. This parallels naturally occurring camelid and shark single-domain antibodies (sdAbs) which are also characterized by (i) solubility and reversible unfolding, (ii) a high occurrence of disulfide forming Cys in their CDRs, particularly, in CDR1 and CDR3 and (iii) acidic V(H)s as inferred here by a pI distribution analysis, reported here, of pools of human and camelid V(H) and V(H)H (camelid heavy chain antibody V(H)) sequences. Our results, reinforced by previous observations by others, suggest that protein acidification may yet be another mechanism nature has devised to create functional sdAbs and that this concept along with the inclusion of inter-CDR disulfide linkages may be applied to human V(H) domains/libraries for non-aggregation optimization. In addition, calculation of theoretical pIs of V(H)s selected by panning may be used for rapid and precise identification of non-aggregating V(H)s." @default.
- W2130398470 created "2016-06-24" @default.
- W2130398470 creator A5007754711 @default.
- W2130398470 creator A5019014799 @default.
- W2130398470 creator A5046010935 @default.
- W2130398470 creator A5078234546 @default.
- W2130398470 creator A5080385404 @default.
- W2130398470 creator A5086486381 @default.
- W2130398470 creator A5087395125 @default.
- W2130398470 date "2008-11-24" @default.
- W2130398470 modified "2023-10-01" @default.
- W2130398470 title "Aggregation-resistant VHs selected by in vitro evolution tend to have disulfide-bonded loops and acidic isoelectric points" @default.
- W2130398470 cites W1568627452 @default.
- W2130398470 cites W1965692673 @default.
- W2130398470 cites W1976784576 @default.
- W2130398470 cites W1977092907 @default.
- W2130398470 cites W1978217631 @default.
- W2130398470 cites W1980308557 @default.
- W2130398470 cites W1984969969 @default.
- W2130398470 cites W1991644988 @default.
- W2130398470 cites W1991888686 @default.
- W2130398470 cites W1992274755 @default.
- W2130398470 cites W1993575340 @default.
- W2130398470 cites W1994287518 @default.
- W2130398470 cites W1994729007 @default.
- W2130398470 cites W2017888871 @default.
- W2130398470 cites W2019486739 @default.
- W2130398470 cites W2020972820 @default.
- W2130398470 cites W2021256658 @default.
- W2130398470 cites W2025827008 @default.
- W2130398470 cites W2029386192 @default.
- W2130398470 cites W2031989831 @default.
- W2130398470 cites W2033079214 @default.
- W2130398470 cites W2037302035 @default.
- W2130398470 cites W2045725488 @default.
- W2130398470 cites W2060913542 @default.
- W2130398470 cites W2062653170 @default.
- W2130398470 cites W2065629634 @default.
- W2130398470 cites W2072902418 @default.
- W2130398470 cites W2077679863 @default.
- W2130398470 cites W2078361457 @default.
- W2130398470 cites W2078936681 @default.
- W2130398470 cites W2083494119 @default.
- W2130398470 cites W2086132576 @default.
- W2130398470 cites W2090916171 @default.
- W2130398470 cites W2091925589 @default.
- W2130398470 cites W2097478805 @default.
- W2130398470 cites W2100891723 @default.
- W2130398470 cites W2104178116 @default.
- W2130398470 cites W2106522450 @default.
- W2130398470 cites W2110739442 @default.
- W2130398470 cites W2132567536 @default.
- W2130398470 cites W2137718908 @default.
- W2130398470 cites W2140060739 @default.
- W2130398470 cites W2140142521 @default.
- W2130398470 cites W2144265524 @default.
- W2130398470 cites W2154104181 @default.
- W2130398470 cites W2155387120 @default.
- W2130398470 cites W2165984662 @default.
- W2130398470 cites W2169563920 @default.
- W2130398470 cites W2170269585 @default.
- W2130398470 cites W2175687390 @default.
- W2130398470 doi "https://doi.org/10.1093/protein/gzn071" @default.
- W2130398470 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19033278" @default.
- W2130398470 hasPublicationYear "2008" @default.
- W2130398470 type Work @default.
- W2130398470 sameAs 2130398470 @default.
- W2130398470 citedByCount "75" @default.
- W2130398470 countsByYear W21303984702012 @default.
- W2130398470 countsByYear W21303984702013 @default.
- W2130398470 countsByYear W21303984702014 @default.
- W2130398470 countsByYear W21303984702015 @default.
- W2130398470 countsByYear W21303984702016 @default.
- W2130398470 countsByYear W21303984702017 @default.
- W2130398470 countsByYear W21303984702018 @default.
- W2130398470 countsByYear W21303984702019 @default.
- W2130398470 countsByYear W21303984702020 @default.
- W2130398470 countsByYear W21303984702021 @default.
- W2130398470 countsByYear W21303984702022 @default.
- W2130398470 countsByYear W21303984702023 @default.
- W2130398470 crossrefType "journal-article" @default.
- W2130398470 hasAuthorship W2130398470A5007754711 @default.
- W2130398470 hasAuthorship W2130398470A5019014799 @default.
- W2130398470 hasAuthorship W2130398470A5046010935 @default.
- W2130398470 hasAuthorship W2130398470A5078234546 @default.
- W2130398470 hasAuthorship W2130398470A5080385404 @default.
- W2130398470 hasAuthorship W2130398470A5086486381 @default.
- W2130398470 hasAuthorship W2130398470A5087395125 @default.
- W2130398470 hasBestOaLocation W21303984701 @default.
- W2130398470 hasConcept C104317684 @default.
- W2130398470 hasConcept C12554922 @default.
- W2130398470 hasConcept C153911025 @default.
- W2130398470 hasConcept C159654299 @default.
- W2130398470 hasConcept C167625842 @default.
- W2130398470 hasConcept C181199279 @default.
- W2130398470 hasConcept C185592680 @default.
- W2130398470 hasConcept C27256138 @default.
- W2130398470 hasConcept C2777820669 @default.