Matches in SemOpenAlex for { <https://semopenalex.org/work/W2130513979> ?p ?o ?g. }
- W2130513979 endingPage "4243" @default.
- W2130513979 startingPage "4233" @default.
- W2130513979 abstract "The proliferating-cell nuclear antigen (PCNA) gene encodes an auxiliary factor of DNA polymerase delta and functions in DNA replication during S phase. It is expressed at much higher levels in proliferating cells than in quiescent cells. We have studied the regulatory role of the 5'-flanking sequence of the murine PCNA gene in interleukin 2 (IL-2)-responsive cloned T cells (L2). Analysis of a set of deletion constructs in transient transfection assays measuring heterologous reporter gene (luciferase) activity demonstrated that the 182-bp 5'-flanking region provides full promoter activity in IL-2-stimulated L2 cells. While many elements contribute to PCNA promoter strength in IL-2-stimulated cells, the largest decrease in activity occurred with deletion of the tandem CRE (cyclic AMP response element) binding sites located at nucleotides -37 to -52. With a gel mobility shift assay, several IL-2-inducible DNA-protein complexes were detected, including CREB (CRE-binding) and ATF1 (activating transcription factor) proteins that are specific for the PCNA-CRE sequence. Methylation interference analysis confirmed specific binding of these proteins to the CRE sites. Mutation at the PCNA-CRE motif abolishes IL-2-inducible binding and reduces substantially PCNA promoter activity. These results indicate that IL-2-stimulated PCNA transcription may be partially mediated by these CRE-binding proteins." @default.
- W2130513979 created "2016-06-24" @default.
- W2130513979 creator A5047490324 @default.
- W2130513979 creator A5055412548 @default.
- W2130513979 creator A5081513362 @default.
- W2130513979 creator A5085800731 @default.
- W2130513979 creator A5088560851 @default.
- W2130513979 date "1994-06-01" @default.
- W2130513979 modified "2023-09-27" @default.
- W2130513979 title "Promoter activity of the proliferating-cell nuclear antigen gene is associated with inducible CRE-binding proteins in interleukin 2-stimulated T lymphocytes." @default.
- W2130513979 cites W1221384716 @default.
- W2130513979 cites W123775848 @default.
- W2130513979 cites W1497850848 @default.
- W2130513979 cites W1503849321 @default.
- W2130513979 cites W1506869135 @default.
- W2130513979 cites W1509615239 @default.
- W2130513979 cites W1525913425 @default.
- W2130513979 cites W1550698494 @default.
- W2130513979 cites W1564556915 @default.
- W2130513979 cites W1569934864 @default.
- W2130513979 cites W1589231329 @default.
- W2130513979 cites W1601085105 @default.
- W2130513979 cites W1620091220 @default.
- W2130513979 cites W1806844342 @default.
- W2130513979 cites W1811606715 @default.
- W2130513979 cites W1824945302 @default.
- W2130513979 cites W1843163606 @default.
- W2130513979 cites W1846573294 @default.
- W2130513979 cites W1884174895 @default.
- W2130513979 cites W1884777442 @default.
- W2130513979 cites W1919026068 @default.
- W2130513979 cites W1956097919 @default.
- W2130513979 cites W1965267927 @default.
- W2130513979 cites W1967004332 @default.
- W2130513979 cites W1967494967 @default.
- W2130513979 cites W1967880575 @default.
- W2130513979 cites W1973135307 @default.
- W2130513979 cites W1974391793 @default.
- W2130513979 cites W1988831637 @default.
- W2130513979 cites W1988991132 @default.
- W2130513979 cites W1989153321 @default.
- W2130513979 cites W1993243592 @default.
- W2130513979 cites W1995129870 @default.
- W2130513979 cites W1998844870 @default.
- W2130513979 cites W1999876280 @default.
- W2130513979 cites W2000024512 @default.
- W2130513979 cites W2000324336 @default.
- W2130513979 cites W2001230855 @default.
- W2130513979 cites W2003330976 @default.
- W2130513979 cites W2010105075 @default.
- W2130513979 cites W2012913493 @default.
- W2130513979 cites W2031504305 @default.
- W2130513979 cites W2032210572 @default.
- W2130513979 cites W2033371710 @default.
- W2130513979 cites W2035537276 @default.
- W2130513979 cites W2037777120 @default.
- W2130513979 cites W2038149991 @default.
- W2130513979 cites W2041079932 @default.
- W2130513979 cites W2043261593 @default.
- W2130513979 cites W2045590352 @default.
- W2130513979 cites W2049020751 @default.
- W2130513979 cites W2051770415 @default.
- W2130513979 cites W2055162988 @default.
- W2130513979 cites W2058692804 @default.
- W2130513979 cites W2070697949 @default.
- W2130513979 cites W2074351361 @default.
- W2130513979 cites W2074745312 @default.
- W2130513979 cites W2076063939 @default.
- W2130513979 cites W2081704409 @default.
- W2130513979 cites W2085585711 @default.
- W2130513979 cites W2094807913 @default.
- W2130513979 cites W2097794242 @default.
- W2130513979 cites W2100747076 @default.
- W2130513979 cites W2109399660 @default.
- W2130513979 cites W2112005597 @default.
- W2130513979 cites W2112941906 @default.
- W2130513979 cites W2136210811 @default.
- W2130513979 cites W2144634347 @default.
- W2130513979 cites W2148231718 @default.
- W2130513979 cites W2154109112 @default.
- W2130513979 cites W2155261977 @default.
- W2130513979 cites W2163976338 @default.
- W2130513979 cites W2171367003 @default.
- W2130513979 cites W2171975928 @default.
- W2130513979 cites W49383898 @default.
- W2130513979 doi "https://doi.org/10.1128/mcb.14.6.4233" @default.
- W2130513979 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/358789" @default.
- W2130513979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7910946" @default.
- W2130513979 hasPublicationYear "1994" @default.
- W2130513979 type Work @default.
- W2130513979 sameAs 2130513979 @default.
- W2130513979 citedByCount "28" @default.
- W2130513979 countsByYear W21305139792012 @default.
- W2130513979 countsByYear W21305139792013 @default.
- W2130513979 crossrefType "journal-article" @default.
- W2130513979 hasAuthorship W2130513979A5047490324 @default.
- W2130513979 hasAuthorship W2130513979A5055412548 @default.
- W2130513979 hasAuthorship W2130513979A5081513362 @default.