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- W2130583703 abstract "The use of a properly selected activating solution during artificial fertilization permits improvement in spermatozoa motility characteristics and enhancement of the fertilization success. This is particularly important for species characterised by low motility parameters. The aim of the study was to determine the types of environments favourable for whitefish (Coregonus lavaretus L.) spermatozoa activation on the basis of analysis of the spermatozoa motility parameters. Motility parameters were evaluated for four activation media: 30 mm NaCl, Billard's solution, 120 mm NaHCO3 and hatchery water, using computer assisted sperm analysis (CASA). The study was divided into two experiments: (i) in the first, the effects of three solutions were tested: 30 mm NaCl, Billard's solution and 120 mm NaHCO3; (ii) in the second, the effects of hatchery water and 30 mm NaCl were compared. The durations of the active motility phase in the three tested solutions were similar (approximately 150 s), while that measured in hatchery water was significantly shorter (115 s). Upon activation, motility (MOT) was similar in all activating media but the highest values of curvilinear velocity (VCL), straight line velocity (VSL) and amplitude of lateral head displacement (ALH) were observed in 30 mm NaCl. NaCl solution was a suitable environment for the whitefish spermatozoa activation. The velocity parameters obtained in this medium exceed those noted in other tested environments, particularly upon activation." @default.
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- W2130583703 date "2015-05-08" @default.
- W2130583703 modified "2023-09-27" @default.
- W2130583703 title "Spermatozoa motility traits influenced by activation media in the European whitefish (<i>Coregonus lavaretus</i>L. 1758)" @default.
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- W2130583703 doi "https://doi.org/10.1111/jai.12722" @default.
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