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- W2130608434 abstract "Miri, in the north of Sarawak in 2007. Stool samples collected from these two isolated outbreaks were tested for rotavirus, which is a primary etiological agent of gastroenteritis. This study aimed to characterize the G (VP7) and P (VP4) genotypes of group A human rotavirus circulating in these two outbreaks. The G and P types of the rotavirus-positive faecal specimens were determined by reverse-transcription PCR and sequencing. Methods: Faecal specimens were resuspended in PBS and were subjected to nucleic acid extraction by using the Chemagic Viral Extraction Kit (Chemagen, Germany). Rotavirus was detected by RT-PCR using published primers specific for VP4 and VP7. Positive samples were subjected to DNA sequencing and partial sequences of the G and P gene were analysed using Lasergene (DNASTAR, Madison WI, USA) software and phylogenetic tree analysis using a neighbourjoining method. Results: A total of 272 samples were tested for rotavirus and of these 75 (72%) and 68 (40%) in Serian and Miri respectively, were positive for group A human rotavirus. A total of 105 sequences were obtained, and five different G-P combinations were identified. In both outbreaks, G1[P8] were the most common strains found which constituted of 87.6% of all the rotavirus typed. Other genotypes include the emergent strains G9[P8] (7.6%), G2[P4] (3.8%) and a rare G3[P9] (1%). Out of the 5 combinations only G1[P8] and G9[P8] strains were identified in the 2001 outbreak in Serian while G1[P8], G2[P4] and G3[P9] were found circulating in the 2007 outbreak in Miri. Conclusion: Given variations observed in circulating rotavirus strains, continued monitoring of genotypes is essential to identify emerging strains, track common strains and evaluate impact of rotavirus vaccines." @default.
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- W2130608434 date "2008-12-01" @default.
- W2130608434 modified "2023-09-27" @default.
- W2130608434 title "Phylogenetic Analysis of the VP1 Gene of Human Echovirus 30 Isolates from Sarawak Reveals An Emerging Genotype" @default.
- W2130608434 doi "https://doi.org/10.1016/j.ijid.2008.05.230" @default.
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