Matches in SemOpenAlex for { <https://semopenalex.org/work/W2130625221> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2130625221 endingPage "6" @default.
- W2130625221 startingPage "320" @default.
- W2130625221 abstract "This work aimed to detect Campylobacter species from naturally contaminated turbid pond water by PCR. A total of 16 water samples were collected from a turbid village pond. Four methods of DNA extraction were applied to centrifuge pellets from eight 100 ml pond water samples prior to attempted detection of Campylobacter by PCR without an enrichment step. These methods were (1) Tris-HCl and sodium dodecyl sulfate followed by phenol:chloroform:isoamylalcohol extraction followed by treatment with DNA clean up kit, (2) proteinase K, (3) Chelex® 100, and (4) boiling. The other eight pond water samples (10 ml and 100 ml) were filtered and filters were incubated overnight in Preston enrichment broth. The centrifuge pellets obtained from enrichment cultures were treated by proteinase K for DNA extraction. Primers CF03 and CF04 for the flagellin genes (flaA and flaB) of Campylobacter jejuni and Campylobacter coli were used for amplifying the extracted DNA. The DNA extracted from eight-100 ml pond water samples that were not subject to selective enrichment was never amplified with primers CF03 and CF04, hence Campylobacter was not detected. In contrast, the DNA that was from samples that were subjected to a selective enrichment step in Preston broth prior to PCR assay always gave amplified bands of 340-380 bp, therefore the presence of Campylobacter was confirmed. Detection of campylobacters from naturally contaminated, turbid, environmental water may not be feasible by direct PCR assay because of low numbers and the presence of high concentration of humic matter and other PCR inhibitors. The enrichment of water samples in selective broth, however, facilitated PCR detection of Campylobacter probably by increasing cell number and by diluting PCR inhibitors." @default.
- W2130625221 created "2016-06-24" @default.
- W2130625221 creator A5006828374 @default.
- W2130625221 creator A5028281845 @default.
- W2130625221 creator A5040461189 @default.
- W2130625221 date "2014-06-01" @default.
- W2130625221 modified "2023-09-23" @default.
- W2130625221 title "A pre-enrichment step is essential for detection of Campylobacter sp. in turbid pond water." @default.
- W2130625221 cites W1515985097 @default.
- W2130625221 cites W1589349144 @default.
- W2130625221 cites W1979879191 @default.
- W2130625221 cites W2010009199 @default.
- W2130625221 cites W2020803196 @default.
- W2130625221 cites W2027969059 @default.
- W2130625221 cites W2030540762 @default.
- W2130625221 cites W2030827737 @default.
- W2130625221 cites W2037905322 @default.
- W2130625221 cites W2048417342 @default.
- W2130625221 cites W2093283037 @default.
- W2130625221 cites W2102055728 @default.
- W2130625221 cites W2118537438 @default.
- W2130625221 cites W2131512581 @default.
- W2130625221 cites W2132301542 @default.
- W2130625221 cites W2132765483 @default.
- W2130625221 cites W2141514817 @default.
- W2130625221 cites W2170453968 @default.
- W2130625221 cites W2410644002 @default.
- W2130625221 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25134901" @default.
- W2130625221 hasPublicationYear "2014" @default.
- W2130625221 type Work @default.
- W2130625221 sameAs 2130625221 @default.
- W2130625221 citedByCount "1" @default.
- W2130625221 countsByYear W21306252212017 @default.
- W2130625221 crossrefType "journal-article" @default.
- W2130625221 hasAuthorship W2130625221A5006828374 @default.
- W2130625221 hasAuthorship W2130625221A5028281845 @default.
- W2130625221 hasAuthorship W2130625221A5040461189 @default.
- W2130625221 hasConcept C104317684 @default.
- W2130625221 hasConcept C185592680 @default.
- W2130625221 hasConcept C2776957140 @default.
- W2130625221 hasConcept C2777403496 @default.
- W2130625221 hasConcept C2778471508 @default.
- W2130625221 hasConcept C2779910956 @default.
- W2130625221 hasConcept C43617362 @default.
- W2130625221 hasConcept C4725764 @default.
- W2130625221 hasConcept C49105822 @default.
- W2130625221 hasConcept C523546767 @default.
- W2130625221 hasConcept C54355233 @default.
- W2130625221 hasConcept C552990157 @default.
- W2130625221 hasConcept C55493867 @default.
- W2130625221 hasConcept C78063203 @default.
- W2130625221 hasConcept C86803240 @default.
- W2130625221 hasConcept C89423630 @default.
- W2130625221 hasConceptScore W2130625221C104317684 @default.
- W2130625221 hasConceptScore W2130625221C185592680 @default.
- W2130625221 hasConceptScore W2130625221C2776957140 @default.
- W2130625221 hasConceptScore W2130625221C2777403496 @default.
- W2130625221 hasConceptScore W2130625221C2778471508 @default.
- W2130625221 hasConceptScore W2130625221C2779910956 @default.
- W2130625221 hasConceptScore W2130625221C43617362 @default.
- W2130625221 hasConceptScore W2130625221C4725764 @default.
- W2130625221 hasConceptScore W2130625221C49105822 @default.
- W2130625221 hasConceptScore W2130625221C523546767 @default.
- W2130625221 hasConceptScore W2130625221C54355233 @default.
- W2130625221 hasConceptScore W2130625221C552990157 @default.
- W2130625221 hasConceptScore W2130625221C55493867 @default.
- W2130625221 hasConceptScore W2130625221C78063203 @default.
- W2130625221 hasConceptScore W2130625221C86803240 @default.
- W2130625221 hasConceptScore W2130625221C89423630 @default.
- W2130625221 hasIssue "2" @default.
- W2130625221 hasLocation W21306252211 @default.
- W2130625221 hasOpenAccess W2130625221 @default.
- W2130625221 hasPrimaryLocation W21306252211 @default.
- W2130625221 hasRelatedWork W1558520874 @default.
- W2130625221 hasRelatedWork W1860078555 @default.
- W2130625221 hasRelatedWork W2075618213 @default.
- W2130625221 hasRelatedWork W2130625221 @default.
- W2130625221 hasRelatedWork W2163832303 @default.
- W2130625221 hasRelatedWork W2167336521 @default.
- W2130625221 hasRelatedWork W2249045702 @default.
- W2130625221 hasRelatedWork W2442640404 @default.
- W2130625221 hasRelatedWork W2524043121 @default.
- W2130625221 hasRelatedWork W3026559652 @default.
- W2130625221 hasVolume "31" @default.
- W2130625221 isParatext "false" @default.
- W2130625221 isRetracted "false" @default.
- W2130625221 magId "2130625221" @default.
- W2130625221 workType "article" @default.