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• W2130688888 endingPage "1780" @default.
• W2130688888 startingPage "1774" @default.
• W2130688888 abstract "Sperm from C57BL/6J, DBA/2J, BALB/cJ, 129S3/SvImJ, and FVB/NJ inbred mice were cryopreserved in 3% skim milk/18% raffinose cryoprotectant solution. The post-thaw sperm from all strains were evaluated for their viability and fertility by comparing them against B6D2F1 sperm used as a control. The protocol used for freezing mouse sperm was effective in different strains, because the motility was decreased by 50% after cryopreservation similar to other mammalian sperm. However, the progressive motility and the fertility of each inbred strain were affected differently. The C57BL/6J, BALB/cJ, and 129S3/SvImJ strains were the most affected; their fertility (two-cell cleavage) decreased from 70%, 34%, and 84% when using freshly collected sperm to 6%, 12%, and 6% when using frozen/thawed sperm, respectively. Live newborns derived from frozen/thawed sperm were obtained from all strains in the study. These results corroborate the genetic variation among strains with regard to fertility and susceptibility to cryopreservation." @default.
• W2130688888 created "2016-06-24" @default.
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• W2130688888 date "2000-12-01" @default.
• W2130688888 modified "2023-10-06" @default.
• W2130688888 title "In Vitro Fertilization with Cryopreserved Inbred Mouse Sperm1" @default.
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• W2130688888 doi "https://doi.org/10.1095/biolreprod63.6.1774" @default.
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