Matches in SemOpenAlex for { <https://semopenalex.org/work/W2130732011> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2130732011 endingPage "523" @default.
- W2130732011 startingPage "513" @default.
- W2130732011 abstract "Transmission of Caprine Arthritis Encephalitis virus (CAEV) from the mother to offspring is principally mediated by infected cells from colostrum and milk. The infection of the dam is often sub-clinical, and results in increased cellularity of milk, sometimes exacerbated by bacterial co-infections. Although monocytes are the major viral host cells, several other cell types, including epithelial mammary cells, fibroblasts and endothelial cells show low levels of in vivo infection. In vitro, however, all phenotypes of mammary gland cells are individually highly sensitive to CAEV infection. This suggests that local mechanisms act to control viral expression. Our goal is to analyse the mechanisms regulating local virus infection, including the physiological status of the mammary gland and bacterial co-infections. In this work, we present the development of a model for the in vitro reconstitution of mammary gland tissue using 3D cultures in Matrigel. Mononuclear cells from the blood are added to the 3D cultures in vitro. In these experimental conditions, the mammary cells spontaneously organize into mammospheres. Blood leucocytes migrate into the culture gel, and localize particularly at the periphery of the mammospheres. Mammospheres were susceptible to infection in vitro by CAEV, as shown by a cytopathic effect and expression of late CAEV antigen p30. This model will allow the in vitro study of virus expression, transfer of infection to mammary gland cells and interactions between the mammary gland cells, infected monocytes and immunocompetent cells. It will allow the study of mechanisms participating in the control of passage of pathogens into milk, according to the physiological and CAEV-infection status of the animal, microenvironment and the presence of bacterial co-infections." @default.
- W2130732011 created "2016-06-24" @default.
- W2130732011 creator A5004791412 @default.
- W2130732011 creator A5042150539 @default.
- W2130732011 creator A5042883894 @default.
- W2130732011 creator A5046108425 @default.
- W2130732011 date "2005-07-01" @default.
- W2130732011 modified "2023-09-26" @default.
- W2130732011 title "Mammary transmission of caprine arthritis encephalitis virus: a 3D model for in vitro study" @default.
- W2130732011 cites W1814052452 @default.
- W2130732011 cites W1819431653 @default.
- W2130732011 cites W1969600377 @default.
- W2130732011 cites W1980039397 @default.
- W2130732011 cites W2006919434 @default.
- W2130732011 cites W2046998958 @default.
- W2130732011 cites W2047266588 @default.
- W2130732011 cites W2058045552 @default.
- W2130732011 cites W2065372649 @default.
- W2130732011 cites W2090135858 @default.
- W2130732011 cites W2105767082 @default.
- W2130732011 cites W2106354448 @default.
- W2130732011 cites W2142603344 @default.
- W2130732011 cites W2155873106 @default.
- W2130732011 cites W2163639831 @default.
- W2130732011 cites W2177450656 @default.
- W2130732011 doi "https://doi.org/10.1051/rnd:2005035" @default.
- W2130732011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16045898" @default.
- W2130732011 hasPublicationYear "2005" @default.
- W2130732011 type Work @default.
- W2130732011 sameAs 2130732011 @default.
- W2130732011 citedByCount "13" @default.
- W2130732011 countsByYear W21307320112012 @default.
- W2130732011 countsByYear W21307320112015 @default.
- W2130732011 countsByYear W21307320112016 @default.
- W2130732011 countsByYear W21307320112017 @default.
- W2130732011 countsByYear W21307320112020 @default.
- W2130732011 countsByYear W21307320112021 @default.
- W2130732011 countsByYear W21307320112022 @default.
- W2130732011 crossrefType "journal-article" @default.
- W2130732011 hasAuthorship W2130732011A5004791412 @default.
- W2130732011 hasAuthorship W2130732011A5042150539 @default.
- W2130732011 hasAuthorship W2130732011A5042883894 @default.
- W2130732011 hasAuthorship W2130732011A5046108425 @default.
- W2130732011 hasBestOaLocation W21307320111 @default.
- W2130732011 hasConcept C121608353 @default.
- W2130732011 hasConcept C137061746 @default.
- W2130732011 hasConcept C159047783 @default.
- W2130732011 hasConcept C202751555 @default.
- W2130732011 hasConcept C203014093 @default.
- W2130732011 hasConcept C2522874641 @default.
- W2130732011 hasConcept C2778001805 @default.
- W2130732011 hasConcept C530470458 @default.
- W2130732011 hasConcept C54355233 @default.
- W2130732011 hasConcept C55493867 @default.
- W2130732011 hasConcept C86803240 @default.
- W2130732011 hasConceptScore W2130732011C121608353 @default.
- W2130732011 hasConceptScore W2130732011C137061746 @default.
- W2130732011 hasConceptScore W2130732011C159047783 @default.
- W2130732011 hasConceptScore W2130732011C202751555 @default.
- W2130732011 hasConceptScore W2130732011C203014093 @default.
- W2130732011 hasConceptScore W2130732011C2522874641 @default.
- W2130732011 hasConceptScore W2130732011C2778001805 @default.
- W2130732011 hasConceptScore W2130732011C530470458 @default.
- W2130732011 hasConceptScore W2130732011C54355233 @default.
- W2130732011 hasConceptScore W2130732011C55493867 @default.
- W2130732011 hasConceptScore W2130732011C86803240 @default.
- W2130732011 hasIssue "4" @default.
- W2130732011 hasLocation W21307320111 @default.
- W2130732011 hasLocation W21307320112 @default.
- W2130732011 hasLocation W21307320113 @default.
- W2130732011 hasLocation W21307320114 @default.
- W2130732011 hasLocation W21307320115 @default.
- W2130732011 hasOpenAccess W2130732011 @default.
- W2130732011 hasPrimaryLocation W21307320111 @default.
- W2130732011 hasRelatedWork W1487857142 @default.
- W2130732011 hasRelatedWork W1568848974 @default.
- W2130732011 hasRelatedWork W1976124441 @default.
- W2130732011 hasRelatedWork W1988749276 @default.
- W2130732011 hasRelatedWork W2002387064 @default.
- W2130732011 hasRelatedWork W2039910870 @default.
- W2130732011 hasRelatedWork W2056473717 @default.
- W2130732011 hasRelatedWork W2126152546 @default.
- W2130732011 hasRelatedWork W2278836635 @default.
- W2130732011 hasRelatedWork W2313414779 @default.
- W2130732011 hasVolume "45" @default.
- W2130732011 isParatext "false" @default.
- W2130732011 isRetracted "false" @default.
- W2130732011 magId "2130732011" @default.
- W2130732011 workType "article" @default.