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• W2130756502 abstract "Abstract The physiological approach suggests that an environment associating the mesenchymal stromal cells (MSC) and low O 2 concentration would be most favorable for the maintenance of hematopoietic stem cells (HSCs) in course of ex vivo expansion of hematopoietic grafts. To test this hypothesis, we performed a co‐culture of cord blood CD34 + cells with or without MSC in presence of cytokines for 10 days at 20%, 5%, and 1.5% O 2 and assessed the impact on total cells, CD34 + cells, committed progenitors (colony‐forming cells—CFC) and stem cells activity (pre‐CFC and Scid repopulating cells—SRC). Not surprisingly, the expansion of total cells, CD34 + cells, and CFC was higher in co‐culture and at 20% O 2 compared to simple culture and low O 2 concentrations, respectively. However, co‐culture at low O 2 concentrations provided CD34 + cell and CFC amplification similar to classical culture at 20% O 2 . Interestingly, low O 2 concentrations ensured a better pre‐CFC and SRC preservation/expansion in co‐culture. Indeed, SRC activity in co‐culture at 1.5% O 2 was higher than in freshly isolated CD34 + cells. Interleukin‐6 production by MSC at physiologically low O 2 concentrations might be one of the factors mediating this effect. Our data demonstrate that association of co‐culture and low O 2 concentration not only induces sufficient expansion of committed progenitors (with respect to the classical culture), but also ensures a better maintenance/expansion of hematopoietic stem cells (HSCs), pointing to the oxygenation as a physiological regulatory factor but also as a cell engineering tool. J. Cell. Physiol. 227: 2750–2758, 2012. © 2011 Wiley Periodicals, Inc." @default.
• W2130756502 created "2016-06-24" @default.
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• W2130756502 date "2012-02-24" @default.
• W2130756502 modified "2023-10-10" @default.
• W2130756502 title "Combination of low O₂concentration and mesenchymal stromal cells during culture of cord blood CD34⁺cells improves the maintenance and proliferative capacity of hematopoietic stem cells" @default.
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• W2130756502 doi "https://doi.org/10.1002/jcp.23019" @default.
• W2130756502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21913190" @default.
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