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• W2130776312 abstract "A rapid and sensitive postcolumn fluorescence derivatization method was developed for microanalysis of antidiabetic oligosaccharide HS203 in beagle dog plasma. After plasma protein was removed by a simple and fast ultrafiltration method, chromatographic separation was performed on an Asahipak GS-320 HQ column with a mobile phase of 50 mmol/L phosphate buffer (pH 6.7) and acetonitrile (83/17, v/v). The column effluent was monitored by fluorescence detection at 249 nm (excitation) and 435 nm (emission) using guanidine hydrochloride as a postcolumn derivatizing reagent. A satisfactory resolution of the analyte was achieved and the limit of detection was found to be 4 ng (more sensitive than silver staining of HS203 in polyacrylamide gel electrophoresis). The method described above was successfully applied to a pharmacokinetic study of HS203 and to monitor blood glucose level simultaneously in beagle dog. It is also possible to be applied for microanalysis of other oligosaccharides in biological samples." @default.
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• W2130776312 date "2012-06-01" @default.
• W2130776312 modified "2023-10-06" @default.
• W2130776312 title "Microanalysis of oligosaccharide HS203 in beagle dog plasma by postcolumn fluorescence derivatization method" @default.
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• W2130776312 doi "https://doi.org/10.1016/j.carbpol.2012.03.069" @default.
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