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- W2130813680 abstract "A Gram-negative, rod-shaped, motile, aerobic bacterial strain, W3 T , was isolated from hexachlorocyclohexane (HCH)-contaminated groundwater from Lucknow, India, and its taxonomic position was determined using a polyphasic approach. Strain W3 T shared highest 16S rRNA gene sequence similarity of 97.8 % with Rhizobium selenitireducens B1 T , followed by Rhizobium daejeonense L61 T (97.7 %), Rhizobium radiobacter ATCC 19358 T (97.5 %) and Blastobacter aggregatus IFAM 1003 T (97.2 %). Strain W3 T formed a monophyletic clade with Blastobacter aggregatus IFAM 1003 T ( = DSM 1111 T ) in the cluster of species of the genus Rhizobium . Phylogenetic analyses of strain W3 T using atpD and recA gene sequences confirmed the phylogenetic arrangements obtained by using 16S rRNA gene sequences. Hence, the taxonomic characterization of B. aggregatus DSM 1111 T was also undertaken. Strains W3 T and B. aggregatus DSM 1111 T contained summed feature 8 (18 : 1ω7 c and/or 18 : 1ω6 c ; 65.4 and 70.8 %, respectively) as the major fatty acid, characteristic of the genus Rhizobium . DNA–DNA relatedness of strain W3 T with Rhizobium selenitireducens LMG 24075 T , Rhizobium daejeonense DSM 17795 T , Rhizobium radiobacter DSM 30147 T and B. aggregatus DSM 1111 T was 42, 34, 30 and 34 %, respectively. The DNA G+C contents of strain W3 T and B. aggregatus DSM 1111 T were 62.3 and 62.7 mol%, respectively. A nifH gene encoding dinitrogenase reductase was detected in strain W3 T but not in B. aggregatus DSM 1111 T . Based on the results obtained by phylogenetic and chemotaxonomic analyses, phenotypic characterization and DNA–DNA hybridization, it is concluded that strain W3 T represents a novel species of the genus Rhizobium , for which the name Rhizobium rosettiformans sp. nov. is proposed (type strain W3 T = CCM 7583 T = MTCC 9454 T ). It is also proposed that Blastobacter aggregatus Hirsch and Müller 1986 be transferred to the genus Rhizobium as Rhizobium aggregatum comb. nov. (type strain IFAM 1003 T = DSM 1111 T = ATCC 43293 T )." @default.
- W2130813680 created "2016-06-24" @default.
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- W2130813680 date "2011-05-01" @default.
- W2130813680 modified "2023-09-27" @default.
- W2130813680 title "Rhizobium rosettiformans sp. nov., isolated from a hexachlorocyclohexane dump site, and reclassification of Blastobacter aggregatus Hirsch and Müller 1986 as Rhizobium aggregatum comb. nov." @default.
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- W2130813680 doi "https://doi.org/10.1099/ijs.0.017491-0" @default.
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