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• W2130814212 abstract "Hair cells in mouse cochlear cultures are selectively labeled by brief exposure to FM1-43, a styryl dye used to study endocytosis and exocytosis. Real-time confocal microscopy indicates that dye entry is rapid and via the apical surface. Cooling to 4°C and high extracellular calcium both reduce dye loading. Pretreatment with EGTA, a condition that breaks tip links and prevents mechanotransducer channel gating, abolishes subsequent dye loading in the presence of calcium. Dye loading recovers after calcium chelation with a time course similar to that described for tip-link regeneration.<i>Myo7a</i> mutant hair cells, which can transduce but have all mechanotransducer channels normally closed at rest, do not label with FM1-43 unless the bundles are stimulated by large excitatory stimuli. Extracellular perfusion of FM1-43 reversibly blocks mechanotransduction with half-blocking concentrations in the low micromolar range. The block is reduced by high extracellular calcium and is voltage dependent, decreasing at extreme positive and negative potentials, indicating that FM1-43 behaves as a permeant blocker of the mechanotransducer channel. The time course for the relief of block after voltage steps to extreme potentials further suggests that FM1-43 competes with other cations for binding sites within the pore of the channel. FM1-43 does not block the transducer channel from the intracellular side at concentrations that would cause complete block when applied extracellularly. Calcium chelation and FM1-43 both reduce the ototoxic effects of the aminoglycoside antibiotic neomycin sulfate, suggesting that FM1-43 and aminoglycosides enter hair cells via the same pathway." @default.
• W2130814212 created "2016-06-24" @default.
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• W2130814212 date "2001-09-15" @default.
• W2130814212 modified "2023-10-17" @default.
• W2130814212 title "FM1-43 Dye Behaves as a Permeant Blocker of the Hair-Cell Mechanotransducer Channel" @default.
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• W2130814212 doi "https://doi.org/10.1523/jneurosci.21-18-07013.2001" @default.
• W2130814212 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6762973" @default.
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