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• W2130897203 abstract "Abstract Small angle X‐ray scattering (SAXS) is a powerful method for investigating macromolecular structure in solution. SAXS data provide information about the size and shape of a molecule with a resolution of ∼2 to 3 nm. SAXS is particularly useful for the investigation of nucleic acids, which scatter X‐rays strongly due to the electron‐rich phosphate backbone. Therefore, SAXS has become an increasingly popular method for modeling nucleic acid structures, an endeavor made tractable by the highly regular helical nature of nucleic acid secondary structures. Recently, SAXS was used in combination with NMR to filter and refine all‐atom models of a U2/U6 small nuclear RNA complex. In this unit, general protocols for sample preparation, data acquisition, and data analysis and processing are given. Additionally, examples of correctly and incorrectly processed SAXS data and expected results are przovided. Curr. Protoc. Nucleic Acid Chem . 51:7.18.1‐7.18.18. © 2012 by John Wiley & Sons, Inc." @default.
• W2130897203 created "2016-06-24" @default.
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• W2130897203 date "2012-12-01" @default.
• W2130897203 modified "2023-10-10" @default.
• W2130897203 title "Nucleic Acid Structure Characterization by Small Angle X‐Ray Scattering (SAXS)" @default.
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• W2130897203 doi "https://doi.org/10.1002/0471142700.nc0718s51" @default.
• W2130897203 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3552392" @default.
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